Optimizing the affinity and specificity of proteins with molecular display

被引：87

作者：

Levin, AM ^{[1
]}

Weiss, GA ^{[1
]}

机构：

[1] Univ Calif Irvine, Dept Chem, Irvine, CA 92697 USA

来源：

MOLECULAR BIOSYSTEMS | 2006年 / 2卷 / 01期

关键词：

D O I：

10.1039/b511782h

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Affinity maturation of receptor-ligand interactions represents an important area of academic and pharmaceutical research. Improving affinity and specificity of proteins can tailor potency for both in vivo and in vitro, applications. A number of different display platforms including phage display, bacterial and yeast display, ribosome display, and mRNA display can optimize protein affinity and specificity. Here, we will review the advantages and disadvantages of these molecular display methods with a focus on their suitability for protein affinity maturation.

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页码：49 / 57

页数：9

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