Vesicular stomatitis virus pseudotyped with severe acute respiratory syndrome coronavirus spike protein

被引:104
作者
Fukushi, S
Mizutani, T
Saijo, M
Matsuyama, S
Miyajima, N
Taguchi, F
Itamura, S
Kurane, I
Morikawa, S
机构
[1] Natl Inst Infect Dis, Special Pathogens Lab, Dept Virol 1, Lab Resp Viral Dis, Tokyo 2080011, Japan
[2] Natl Inst Infect Dis, SARS, Tokyo 2080011, Japan
[3] Natl Inst Infect Dis, Lab Influenza Virus, Dept Virol 3, Tokyo 2080011, Japan
关键词
D O I
10.1099/vir.0.80955-0
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Severe acute respiratory syndrome coronavirus (SARS-CoV) contains a single spike (S) protein, which binds to its receptor, angiotensin-converting enzyme 2 (ACE2), induces membrane fusion and serves as a neutralizing antigen. A SARS-CoV-S protein-bearing vesicular stomatitis virus (VSV) pseudotype using the VSV Delta G* system was generated. Partial deletion of the SARS-CoV-S protein cytoplasmic domain allowed efficient incorporation into VSV particles and led to the generation of a pseudotype (VSV-SARS-St19) at high titre. Green fluorescent protein expression was demonstrated as early as 7 In after infection of Vero E6 cells with VSV-SARS-St19. VSV-SARS-St19 was neutralized by anti-SARS-CoV antibody and soluble ACE2, and its infection was blocked by treatment of Vero E6 cells with anti-ACE2 antibody. These results indicated that VSV-SARS-St19 infection is mediated by SARS-CoV-S protein in an ACE2-dependent manner. VSV-SARS-St19 will be useful for analysing the function of SARS-CoV-S protein and for developing rapid methods of detecting neutralizing antibodies specific for SARS-CoV infection.
引用
收藏
页码:2269 / 2274
页数:6
相关论文
共 22 条
  • [1] Identification of a novel coronavirus in patients with severe acute respiratory syndrome
    Drosten, C
    Günther, S
    Preiser, W
    van der Werf, S
    Brodt, HR
    Becker, S
    Rabenau, H
    Panning, M
    Kolesnikova, L
    Fouchier, RAM
    Berger, A
    Burguière, AM
    Cinatl, J
    Eickmann, M
    Escriou, N
    Grywna, K
    Kramme, S
    Manuguerra, JC
    Müller, S
    Rickerts, V
    Stürmer, M
    Vieth, S
    Klenk, HD
    Osterhaus, ADME
    Schmitz, H
    Doerr, HW
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 2003, 348 (20) : 1967 - 1976
  • [2] Aetiology -: Koch's postulates fulfilled for SARS virus
    Fouchier, RAM
    Kuiken, T
    Schutten, M
    van Amerongen, G
    van Doornum, J
    van den Hoogen, BG
    Peiris, M
    Lim, W
    Stöhr, K
    Osterhaus, ADME
    [J]. NATURE, 2003, 423 (6937) : 240 - 240
  • [3] Retroviral vectors pseudotyped with severe acute respiratory syndrome coronavirus S protein
    Giroglou, T
    Cinatl, J
    Rabenau, H
    Drosten, C
    Schwalbe, H
    Doerr, HW
    von Laer, D
    [J]. JOURNAL OF VIROLOGY, 2004, 78 (17) : 9007 - 9015
  • [4] Susceptibility to SARS coronavirus S protein-driven infection correlates with expression of angiotensin converting enzyme 2 and infection can be blocked by soluble receptor
    Hofmann, H
    Geier, M
    Marzi, A
    Krumbiegel, M
    Peipp, M
    Fey, GH
    Gramberg, T
    Pöhlmann, S
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2004, 319 (04) : 1216 - 1221
  • [5] Novel peptide inhibitors of angiotensin-converting enzyme 2
    Huang, LL
    Sexton, DJ
    Skogerson, K
    Devlin, M
    Smith, R
    Sanyal, I
    Parry, T
    Kent, R
    Enright, J
    Wu, QL
    Conley, G
    DeOliveira, D
    Morganelli, L
    Ducar, M
    Wescott, CR
    Ladner, RC
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (18) : 15532 - 15540
  • [6] Molecular cloning, mRNA expression and chromosomal localization of mouse angiotensin-converting enzyme-related carboxypeptidase (mACE2)
    Komatsu, T
    Suzuki, Y
    Imai, J
    Sugano, S
    Hida, M
    Tanigami, A
    Muroi, S
    Yamada, Y
    Hanaoka, K
    [J]. DNA SEQUENCE, 2002, 13 (04): : 217 - 220
  • [7] A novel coronavirus associated with severe acute respiratory syndrome
    Ksiazek, TG
    Erdman, D
    Goldsmith, CS
    Zaki, SR
    Peret, T
    Emery, S
    Tong, SX
    Urbani, C
    Comer, JA
    Lim, W
    Rollin, PE
    Dowell, SF
    Ling, AE
    Humphrey, CD
    Shieh, WJ
    Guarner, J
    Paddock, CD
    Rota, P
    Fields, B
    DeRisi, J
    Yang, JY
    Cox, N
    Hughes, JM
    LeDuc, JW
    Bellini, WJ
    Anderson, LJ
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 2003, 348 (20) : 1953 - 1966
  • [8] Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus
    Li, WH
    Moore, MJ
    Vasilieva, N
    Sui, JH
    Wong, SK
    Berne, MA
    Somasundaran, M
    Sullivan, JL
    Luzuriaga, K
    Greenough, TC
    Choe, H
    Farzan, M
    [J]. NATURE, 2003, 426 (6965) : 450 - 454
  • [9] The genome sequence of the SARS-associated coronavirus
    Marra, MA
    Jones, SJM
    Astell, CR
    Holt, RA
    Brooks-Wilson, A
    Butterfield, YSN
    Khattra, J
    Asano, JK
    Barber, SA
    Chan, SY
    Cloutier, A
    Coughlin, SM
    Freeman, D
    Girn, N
    Griffith, OL
    Leach, SR
    Mayo, M
    McDonald, H
    Montgomery, SB
    Pandoh, PK
    Petrescu, AS
    Robertson, AG
    Schein, JE
    Siddiqui, A
    Smailus, DE
    Stott, JE
    Yang, GS
    Plummer, F
    Andonov, A
    Artsob, H
    Bastien, N
    Bernard, K
    Booth, TF
    Bowness, D
    Czub, M
    Drebot, M
    Fernando, L
    Flick, R
    Garbutt, M
    Gray, M
    Grolla, A
    Jones, S
    Feldmann, H
    Meyers, A
    Kabani, A
    Li, Y
    Normand, S
    Stroher, U
    Tipples, GA
    Tyler, S
    [J]. SCIENCE, 2003, 300 (5624) : 1399 - 1404
  • [10] Characterization of pseudotype VSV possessing HCV envelope proteins
    Matsuura, Y
    Tani, H
    Suzuki, K
    Kimura-Someya, T
    Suzuki, R
    Aizaki, H
    Ishii, K
    Moriishi, K
    Robison, CS
    Whitt, MA
    Miyamura, T
    [J]. VIROLOGY, 2001, 286 (02) : 263 - 275