Two- and multiphoton excitation of conjugate-dyes using a continuous wave laser

被引:28
作者
Hanninen, PE
Lehtela, L
Hell, SW
机构
[1] UNIV TURKU,TURKU 20521,FINLAND
[2] CTR BIOTECHNOL,TURKU 20521,FINLAND
关键词
D O I
10.1016/0030-4018(96)00189-7
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
We discuss the possibility of obtaining a fluorescence signal depending nonlinearly on the excitation intensity by inhibiting the fluorescence energy transfer in a conjugate-pair dye molecule and its application in scanning microscopy. The dynamical behaviour of the conjugate dye molecule and the nonlinear behaviour of the fluorescence intensity is analysed. The benefits for scanning fluorescence microscopy are outlined.
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页码:29 / 33
页数:5
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