Production of artemisinin by shoot cultures of Artemisia annua L. in a modified inner-loop mist bioreactor

被引：22

作者：

Liu, CZ ^{[1
]}

Wang, YC

Guo, C

Ouyang, F

Ye, HC

Li, GF

机构：

[1] Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China

[2] Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China

来源：

PLANT SCIENCE | 1998年 / 135卷 / 02期

关键词：

Artemisia annua L; artemisinin; misting cycle; shoot culture; modified inner-loop mist bioreactor;

D O I：

10.1016/S0168-9452(98)00086-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A modified inner-loop ultrasonic nutrient mist bioreactor with three stainless steel meshes was used to culture shoots of Artemisia annua L. for the production of artemisinin. The distribution of nutrient mist and the characteristics of shoot growth in the bioreactor were observed. Under the optimal misting cycle of 3/90 (3 min of misting ON, followed by 90 min of misting OFF), the dry weight of biomass and artemisinin production in the bioreactor reached 13.3 and 46.9 mg/l after 25 days. The growth and artemisinin accumulation in the mist bioreactor were higher than those in flasks. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：211 / 217

页数：7

共 31 条

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