Cell morphology and intracellular ionic homeostasis explored with a multimodal approach combining epifluorescence and digital holographic microscopy

被引：75

作者：

Pavillon, Nicolas ^{[1
]}

Benke, Alexander ^{[2
]}

Boss, Daniel ^{[2
]}

Moratal, Corinne ^{[2
]}

Kuehn, Jonas ^{[3
]}

Jourdain, Pascal ^{[2
]}

Depeursinge, Christian ^{[1
]}

Magistretti, Pierre J. ^{[2
,3
]}

Marquet, Pierre ^{[2
,3
]}

机构：

[1] Ecole Polytech Fed Lausanne, Adv Photon Lab MVD, CH-1015 Lausanne, Switzerland

[2] Ecole Polytech Fed Lausanne, Brain Mind Inst, CH-1015 Lausanne, Switzerland

[3] CHU Vaudois, Dept Psychiat, CH-1008 Lausanne, Switzerland

来源：

JOURNAL OF BIOPHOTONICS | 2010年 / 3卷 / 07期

基金：

瑞士国家科学基金会;

关键词：

optics and photonics; cell biology; interference microscopy; fluorescence microscopy; PHASE-CONTRAST MICROSCOPY; REFRACTIVE-INDEX; LIVING CELLS; MORPHOMETRY; TRACKING;

D O I：

10.1002/jbio.201000018

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The authors have developed a live cell multimodality microscope combining epifluorescence with digital holographic microscopoy; it has been implemented with a de-coupling procedure allowing to separately measure from the quantitative phase important cell parameters including absolute volume, shape and integral intracellular retractive index. In combination with the numerouos different specific fluorescent cellular probes this multimodality microscopy can address important issues in cell biology. This is demosntrated by the study of intracellular calcium homeostasis associated with the change in cell volume, whihc play a critical role in the excitotoxicity induced neuronal death. [GRAPHICS] Comparison between quantitative phase (left) and fluorescence signal (right) on neuronal cellular bodies.

引用

页码：432 / 436

页数：5

共 20 条

[1] Molecular mechanisms underlying specificity of excitotoxic signaling in neurons [J].

Aarts, MM ;

Tymianski, M .

CURRENT MOLECULAR MEDICINE, 2004, 4 (02) :137-147

[2] The role of apoptotic volume decrease and ionic homeostasis in the activation and repression of apoptosis [J].

Bortner, CD ;

Cidlowski, JA .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 2004, 448 (03) :313-318

[3] OPTIMIZED SURVIVAL OF HIPPOCAMPAL-NEURONS IN B27-SUPPLEMENTED NEUROBASAL(TM), A NEW SERUM-FREE MEDIUM COMBINATION [J].

BREWER, GJ ;

TORRICELLI, JR ;

EVEGE, EK ;

PRICE, PJ .

JOURNAL OF NEUROSCIENCE RESEARCH, 1993, 35 (05) :567-576

[4] Parameter-optimized digital holographic microscope for high-resolution living-cell analysis [J].

Carl, D ;

Kemper, B ;

Wernicke, G ;

von Bally, G .

APPLIED OPTICS, 2004, 43 (36) :6536-6544

[5]

CHOI DW, 1994, PROG BRAIN RES, V100, P47

[6] Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms [J].

Cuche, E ;

Marquet, P ;

Depeursinge, C .

APPLIED OPTICS, 1999, 38 (34) :6994-7001

[7] Tracking biological microorganisms in sequence of 3D holographic microscopy images [J].

DaneshPanah, Mehdi ;

Javidi, Bahram .

OPTICS EXPRESS, 2007, 15 (17) :10761-10766

[8] Three-dimensional imaging and processing using computational holographic imaging [J].

Frauel, Y ;

Naughton, TJ ;

Matoba, O ;

Tajahuerce, E ;

Javidi, B .

PROCEEDINGS OF THE IEEE, 2006, 94 (03) :636-653

[9] A NEW MICROSCOPIC PRINCIPLE [J].

GABOR, D .

NATURE, 1948, 161 (4098) :777-778

[10] High-resolution quantitative phase-contrast microscopy by digital holography [J].

Mann, CJ ;

Yu, LF ;

Lo, CM ;

Kim, MK .

OPTICS EXPRESS, 2005, 13 (22) :8693-8698

← 1 2 →