Electrochemical monitoring of in vivo reconstitution of glucose dehydrogenase in Escherichia coli cells with externally added pyrroloquinoline quinone

被引：11

作者：

Ikeda, T ^{[1
]}

Matsubara, H

Kato, K

Iswantini, D

Kano, K

Yamada, M

机构：

[1] Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Sakyo Ku, Kyoto 60601, Japan

[2] Yamaguchi Univ, Fac Agr, Dept Biol Chem, Yamaguchi 753, Japan

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 1998年 / 449卷 / 1-2期

关键词：

mediated electrocatalysis; Escherichia coli cells; glucose dehydrogenase; pyrroloquinoline quinone;

D O I：

10.1016/S0022-0728(98)00052-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Mediated bacterial electrocatalysis is used to follow the activation process of ape-glucose dehydrogenase in Escherichia coli cells. A catalytic current is produced at an electrode modified with the bacterial cells when pyrroloquinoline quinone (PQQ) is added to the test solution containing glucose and an electron acceptor. The current is due to the catalytic action of hole-glucose dehydrogenase which is formed from the ape-enzyme with externally added PQQ The catalytic current measurements reveal that the rate of incorporation of PQQ into the enzyme in the bacterial cells depends on the PQQ concentration in the sample solution and that magnesium ions have a significant effect on the hole-enzyme formation. (C) 1998 Elsevier Science S.A. All rights reserved.

引用

页码：219 / 224

页数：6

共 14 条

[1] PURIFICATION AND CHARACTERIZATION OF THE QUINOPROTEIN D-GLUCOSE DEHYDROGENASE APOENZYME FROM ESCHERICHIA-COLI [J].

AMEYAMA, M ;

NONOBE, M ;

SHINAGAWA, E ;

MATSUSHITA, K ;

TAKIMOTO, K ;

ADACHI, O .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1986, 50 (01) :49-57

[2] METHOD OF ENZYMATIC DETERMINATION OF PYRROLOQUINOLINE QUINONE [J].

AMEYAMA, M ;

NONOBE, M ;

SHINAGAWA, E ;

MATSUSHITA, K ;

ADACHI, O .

ANALYTICAL BIOCHEMISTRY, 1985, 151 (02) :263-267

[3] MODE OF BINDING OF PYRROLOQUINOLINE QUINONE TO APO-GLUCOSE DEHYDROGENASE [J].

AMEYAMA, M ;

NONOBE, M ;

HAYASHI, M ;

SHINAGAWA, E ;

MATSUSHITA, K ;

ADACHI, O .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1985, 49 (04) :1227-1231

[4] EXISTENCE OF A NOVEL PROSTHETIC GROUP, PQQ, IN MEMBRANE-BOUND, ELECTRON-TRANSPORT CHAIN-LINKED, PRIMARY DEHYDROGENASES OF OXIDATIVE BACTERIA [J].

AMEYAMA, M ;

MATSUSHITA, K ;

OHNO, Y ;

SHINAGAWA, E ;

ADACHI, O .

FEBS LETTERS, 1981, 130 (02) :179-183

[5] DETECTION AND DETERMINATION OF PYRROLOQUINOLINE QUINONE, THE CO-ENZYME OF QUINOPROTEINS [J].

DUINE, JA ;

FRANK, J ;

JONGEJAN, JA .

ANALYTICAL BIOCHEMISTRY, 1983, 133 (01) :239-243

[6] GLUCOSE-DEHYDROGENASE FROM ACINETOBACTER-CALCOACETICUS - QUINOPROTEIN [J].

DUINE, JA ;

FRANK, J ;

VANZEELAND, JK .

FEBS LETTERS, 1979, 108 (02) :443-446

[7]

HOMMES RWJ, 1984, FEMS MICROBIOL LETT, V24, P329, DOI 10.1016/0378-1097(84)90274-X

[8] Electrocatalytic properties of Acetobacter aceti cells immobilized on electrodes for the quinone-mediated oxidation of ethanol [J].

Ikeda, T ;

Kato, K ;

Maeda, M ;

Tatsumi, H ;

Kano, K ;

Matsushita, K .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1997, 430 (1-2) :197-204

[9] SENSITIVE YEAST BIOELECTRODE TO L-LACTATE [J].

KULYS, J ;

WANG, LZ ;

RAZUMAS, V .

ELECTROANALYSIS, 1992, 4 (05) :527-532

[10] SOLUBLE AND MEMBRANE-BOUND QUINOPROTEIN D-GLUCOSE DEHYDROGENASES OF THE ACINETOBACTER-CALCOACETICUS - THE BINDING PROCESS OF PQQ TO THE APOENZYMES [J].

MATSUSHITA, K ;

TOYAMA, H ;

AMEYAMA, M ;

ADACHI, O ;

DEWANTI, A ;

DUINE, JA .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1995, 59 (08) :1548-1555

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