A novel accessory role of neutrophils in concanavalin A-induced hepatitis

被引：36

作者：

Hatada, S ^{[1
]}

Ohta, T ^{[1
]}

Shiratsuchi, Y ^{[1
]}

Hatano, M ^{[1
]}

Kobayashi, Y ^{[1
]}

机构：

[1] Toho Univ, Fac Sci, Dept Biomol Sci, Div Mol Med, Chiba 2748510, Japan

来源：

CELLULAR IMMUNOLOGY | 2005年 / 233卷 / 01期

关键词：

IFN-gamma; liver injury; RB6-8C5; T cells; mouse;

D O I：

10.1016/j.cellimm.2005.03.003

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Concanavalin A (Con A)-induced hepatitis has been investigated as a model of T cell-mediated liver injury, in which IFN-gamma plays an essential role by inducing apoptosis of liver cells. Since a large number of neutrophils infiltrate into the liver in the model, the role of neutrophils was investigated in this Study. Con A hardly caused liver injury in neutrophil-depleted mice, as assessed as to the plasma alanine aminotransferase level as well as histochemistry. Neutrophil-depleted mice also failed to produce IFN-gamma. IntracellUlar fFN-gamma staining revealed that, among liver leukocytes, T and NK cells but not neutrophils are the main producers of IFN-gamma. Nylon wool-purified "T cells," however, failed to produce IFN-gamma in response to Con A in vitro, while the production was restored by the addition of neutrophils. Overall, this study suggests that neutrophils play a novel accessory role in IFN-gamma production in Con A-induced hepatitis. (C) 2005 Elsevier Inc. All rights reserved.

引用

页码：23 / 29

页数：7

共 23 条

[21] A T-CELL-DEPENDENT EXPERIMENTAL LIVER-INJURY IN MICE INDUCIBLE BY CONCANAVALIN-A [J].

TIEGS, G ;

HENTSCHEL, J ;

WENDEL, A .

JOURNAL OF CLINICAL INVESTIGATION, 1992, 90 (01) :196-203

[22] Expression of inducible nitric oxide synthase (iNOS/NOS II) in the vestibule of guinea pigs after the application of cisplatin [J].

Watanabe, K ;

Hess, A ;

Bloch, W ;

Michel, O .

ANTI-CANCER DRUGS, 2000, 11 (01) :29-32

[23]

WERMERS GW, 1988, LIVER BIOL PATHOBIOL, P885

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