Sorting of small RNAs into Arabidopsis argonaute complexes is directed by the 5′ terminal nucleotide

被引：1062

作者：

Mi, Shijun ^{[1
]}

Cai, Tao ^{[1
]}

Hu, Yugang ^{[1
]}

Chen, Yemiao ^{[1
]}

Hodges, Emily ^{[2
,3
]}

Ni, Fangrui ^{[1
]}

Wu, Liang ^{[1
]}

Li, Shan ^{[1
]}

Zhou, Huanyu ^{[1
]}

Long, Chengzu ^{[1
]}

Chen, She ^{[1
]}

Hannon, Gregory J. ^{[2
,3
]}

Qi, Yijun ^{[1
]}

机构：

[1] Natl Inst Biol Sci, Beijing 102206, Peoples R China

[2] Watson Sch Biol Sci, Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

[3] Howard Hughes Med Inst, Cold Spring Harbor, NY 11724 USA

来源：

CELL | 2008年 / 133卷 / 01期

关键词：

D O I：

10.1016/j.cell.2008.02.034

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Argonaute (AGO) proteins recruit small RNAs to form the core of RNAi effector complexes. Arabidopsis encodes ten AGO proteins and a large network of small RNAs. How these small RNAs are sorted into specific AGO complexes remains largely unknown. We have cataloged small RNAs resident in four AGO complexes. We found that AGO2 and AGO4 preferentially recruit small RNAs with a 5 ' terminal adenosine, whereas AGO1 harbors microRNAs (miRNAs) that favor a 5 ' terminal uridine. AGO5 predominantly binds small RNAs that initiate with cytosine. Changing the 5 ' terminal nucleotide of an miRNA predictably redirected it into a different AGO complex and alters its biological activity. These results reveal a role for small RNA sequences in assorting among AGO complexes. This suggests that specialization of AGO complexes might involve remodeling the 5 ' end-binding pocket to accept certain small RNA sequences, perhaps explaining the evolutionary drive for miRNAs to initiate with uridine.

引用

页码：116 / 127

页数：12

共 56 条

[1] DRB4-dependent TAS3 trans-acting siRNAs control leaf morphology through AGO7
Adenot, Xavier
Elmayan, Taline
Lauressergues, Dominique
Boutet, Stéphanie
Bouché, Nicolas
Gasciolli, Virginie
Vaucheret, Hervé
[J]. CURRENT BIOLOGY, 2006, 16 (09) : 927 - 932
[2] Allen E, 2005, CELL, V121, P207, DOI 10.1016/j.cell.2005.04.004
[3] MicroRNAs and other tiny endogenous RNAs in C-elegans
Ambros, V
Lee, RC
Lavanway, A
Williams, PT
Jewell, D
[J]. CURRENT BIOLOGY, 2003, 13 (10) : 807 - 818
[4] A novel class of small RNAs bind to MILI protein in mouse testes
Aravin, Alexei
Gaidatzis, Dimos
Pfeffer, Sebastien
Lagos-Quintana, Mariana
Landgraf, Pablo
Iovino, Nicola
Morris, Patricia
Brownstein, Michael J.
Kuramochi-Miyagawa, Satomi
Nakano, Toru
Chien, Minchen
Russo, James J.
Ju, Jingyue
Sheridan, Robert
Sander, Chris
Zavolan, Mihaela
Tuschl, Thomas
[J]. NATURE, 2006, 442 (7099) : 203 - 207
[5] A two-hit trigger for siRNA biogenesis in plants
Axtell, Michael J.
Jan, Calvin
Rajagopalan, Ramya
Bartel, David P.
[J]. CELL, 2006, 127 (03) : 565 - 577
[6] Arabidopsis ARGONAUTE1 is an RNA Slicer that selectively recruits rnicroRNAs and short interfering RNAs
Baumberger, N
Baulcombe, DC
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (33) : 11928 - 11933
[7] AGO1 defines a novel locus of Arabidopsis controlling leaf development
Bohmert, K
Camus, I
Bellini, C
Bouchez, D
Caboche, M
Benning, C
[J]. EMBO JOURNAL, 1998, 17 (01) : 170 - 180
[8] Endogenous siRNAs derived from a pair of natural cis-antisense transcripts regulate salt tolerance in Arabidopsis
Borsani, O
Zhu, JH
Verslues, PE
Sunkar, R
Zhu, JK
[J]. CELL, 2005, 123 (07) : 1279 - 1291
[9] WebLogo: A sequence logo generator
Crooks, GE
Hon, G
Chandonia, JM
Brenner, SE
[J]. GENOME RESEARCH, 2004, 14 (06) : 1188 - 1190
[10] Regulation of AUXIN RESPONSE FACTOR3 by TAS3 ta-siRNA affects developmental timing and patterning in Arabidopsis
Fahlgren, Noah
Montgomery, Talowa A.
Howell, Miya D.
Allen, Edwards
Dvorak, Sarah K.
Alexander, Amanda L.
Carrington, James C.
[J]. CURRENT BIOLOGY, 2006, 16 (09) : 939 - 944

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