A novel fibronectin binding site required for fibronectin fibril growth during matrix assembly

被引:95
作者
Sechler, JL [1 ]
Rao, HW [1 ]
Cumiskey, AM [1 ]
Vega-Colón, I [1 ]
Smith, MS [1 ]
Murata, T [1 ]
Schwarzbauer, JE [1 ]
机构
[1] Princeton Univ, Dept Mol Biol, Princeton, NJ 08544 USA
关键词
fibronectin; matrix assembly; type III repeats; RGD sequence; self-association;
D O I
10.1083/jcb.200102034
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Fibronectin (FN) assembly into a fibrillar extracellular matrix is a stepwise process requiring participation from multiple FN domains. Fibril formation is regulated in part by segments within the first seven type III repeats (III1-7). To define the specific function(s) of this region, recombinant FNs (recFNs) containing an overlapping set of deletions were tested for the ability to assemble into fibrils. Surprisingly, recFN lacking type III repeat III1 (FN Delta III1), which contains a cryptic FN binding site and has been suggested to be essential for fibril assembly, formed a matrix identical in all respects to a native FN matrix. Similarly, displacement of the cell binding domain in repeats III9-10 to a position close to the NH2-terminal assembly domain, as well as a large deletion spanning repeats III4-7, had no effect on assembly. in contrast, two deletions that included repeat III2, Delta III1-2 and Delta III2-5, caused significant reductions in fibril elongation, although binding of FN to the cell surface and initiation of assembly still proceeded. Using individual repeats in binding assays, we show that III2 but not III1 contains an FN binding site. Thus, these results pinpoint repeat III2 as an important module for FN-FN interactions during fibril growth.
引用
收藏
页码:1081 / 1088
页数:8
相关论文
共 34 条
[1]  
AGUIRRE KM, 1994, J BIOL CHEM, V269, P27863
[2]  
ALEXANDER SS, 1979, J BIOL CHEM, V254, P1501
[3]   Fibronectin fibrillogenesis involves the heparin II binding domain of fibronectin [J].
Bultmann, H ;
Santas, AJ ;
Peters, DMP .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (05) :2601-2609
[4]   Formation of sodium dodecyl sulfate-stable fibronectin multimers - Failure to detect products of thiol-disulfide exchange in cyanogen bromide or limited acid digests of stabilized matrix fibronectin [J].
Chen, F ;
Mosher, DF .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (15) :9084-9089
[5]   MONOCLONAL-ANTIBODY TO FIBRONECTIN WHICH INHIBITS EXTRACELLULAR-MATRIX ASSEMBLY [J].
CHERNOUSOV, MA ;
FAERMAN, AI ;
FRID, MG ;
PRINTSEVA, OY ;
KOTELIANSKY, VE .
FEBS LETTERS, 1987, 217 (01) :124-128
[6]  
CHERNOUSOV MA, 1991, J BIOL CHEM, V266, P10851
[8]  
ERICKSON HP, 1983, J BIOL CHEM, V258, P4539
[9]   A novel role for the integrin-binding III-10 module in fibronectin matrix assembly [J].
Hocking, DC ;
Smith, RK ;
McKeownLongo, PJ .
JOURNAL OF CELL BIOLOGY, 1996, 133 (02) :431-444
[10]  
HOCKING DC, 1994, J BIOL CHEM, V269, P19183