7-deoxyloganin 7-hydroxylase in Lonicera japonica cell cultures

被引：13

作者：

Katano, N

Yamamoto, H

Iio, R

Inoue, K

机构：

[1] Gifu Pharmaceut Univ, Dept Pharmacognosy, Gifu 5028585, Japan

[2] Nagasaki Univ, Sch Pharmaceut Sci, Med Plant Garden, Nagasaki 8528521, Japan

来源：

PHYTOCHEMISTRY | 2001年 / 58卷 / 01期

关键词：

Lonicera japonica; Caprifoliaceae; cell suspension cultures; loganin; 7-deoxyloganin; iridoid glucoside; 7-hydroxylase; cytochrome P450; enzyme; biosynthesis;

D O I：

10.1016/S0031-9422(01)00181-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The activity of 7-deoxyloganin 7-hydroxylase, an enzyme catalyzing the conversion of 7-deoxyloganin into loganin, was detected in a microsomal preparation from the cell suspension cultures of Lonicera japonica. It was dependent on NADPH and molecular oxygen. The enzymatic reaction was inhibited by carbon monoxide as well as by several cytochrome P450 inhibitors, especially ketoconazole, indicating that the reaction was mediated by cytochrome P450. The enzyme showed substrate specificity for 7-deoxyloganin. The K-m values for 7-deoxyloganin and NADPH were estimated as 170 and 18 muM, respectively. from Lineweaver-Burk plots. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：53 / 58

页数：6

共 31 条

[1] PREPARATION AND ISOLATION OF DEOXYLOGANIN - ITS ROLE AS PRECURSOR OF LOGANIN AND INDOLE ALKALOIDS [J].