Determination of chitosan in wood and water samples by acidic hydrolysis and liquid chromatography with online fluorescence derivatization

被引：46

作者：

Eikenes, M

Fongen, M

Roed, L

Stenstrom, Y

机构：

[1] Norwegian Forest Res Inst, Dept Wood Tehcnol, N-1432 As, Norway

[2] Amersham Hlth AS, N-0401 Oslo, Norway

[3] Agr Univ Norway, Dept Chem Biotechnol & Food Sci, N-1432 As, Norway

来源：

CARBOHYDRATE POLYMERS | 2005年 / 61卷 / 01期

关键词：

acidic hydrolysis; chitosan; glucosamine; quantification; wood preservation;

D O I：

10.1016/j.carbpol.2005.02.006

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

In recent years chitosans have been investigated as a natural chemical for wood preservation against fungal decay, and chitosan in aqueous solutions has been used in impregnation studies. To evaluate the retention of chitosan after an impregnation process and to evaluate the fixation of chitosan in wood a method for determination of chitosan in wood and water samples has been developed based on acidic hydrolysis of chitosan to glucosamine followed by online derivatization by o-phthalaldehyde, chromatographic separation and fluorescent detection. For wood samples the method was linear up to 45 mg g(-1) chitosan in wood and had a recovery of 86%. The yield of chitosan in water was 87% at 1% (w/v) concentration. (c) 2005 Elsevier Ltd. All fights reserved.

引用

页码：29 / 38

页数：10

共 17 条

[1] Screening of chitosan against wood-deteriorating fungi [J].

Alfredsen, G ;

Eikenes, M ;

Militz, H ;

Solheim, H .

SCANDINAVIAN JOURNAL OF FOREST RESEARCH, 2004, 19 :4-13

[2] A SENSITIVE CHITIN ASSAY FOR THE ESTIMATION OF FUNGAL BIOMASS IN HARDWOODS [J].

BRAID, GH ;

LINE, MA .

HOLZFORSCHUNG, 1981, 35 (01) :10-15

[3]

EIKENES M, IN PRESS J WOOD SCI

[4]

Frederiksen O., 2001, FUNGICID REJER

[5] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC QUANTITATION OF PHENYLTHIOCARBAMYL MURAMIC ACID AND GLUCOSAMINE FROM BACTERIAL-CELL WALLS [J].

HAGEN, SR .

JOURNAL OF CHROMATOGRAPHY, 1993, 632 (1-2) :63-68

[6]

Kobayashi T., 1995, J ANTIBACT ANTIFUNG, V23, P343

[7]

Kobayashi T., 1995, J ANTIBACTERIAL ANTI, V23, P263

[8]

Kumar MNVR, 2000, REACT FUNCT POLYM, V46, P1

[9]

LEE JS, 1993, MOKUZAI GAKKAISHI, V39, P103

[10]

LEE JS, 1992, MOKUZAI GAKKAISHI, V38, P186

← 1 2 →