HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC QUANTITATION OF PHENYLTHIOCARBAMYL MURAMIC ACID AND GLUCOSAMINE FROM BACTERIAL-CELL WALLS

被引：15

作者：

HAGEN, SR

机构：

[1] Ribi ImmunoChem Research, Inc., Hamilton, MT 59840

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 632卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(93)80026-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive assay for the simultaneous determination of muramic acid and glucosamine from bacterial cell walls was developed. Cell wall skeleton (CWS) preparations were hydrolyzed with HCl to liberate free amino sugars, which were subsequently derivatized pre-column with phenylisothiocyanate. The phenylthiocarbamoyl (PTC) amino sugars were rapidly resolved by reversed-phase gradient HPLC and quantified with UV detection. With this assay, Mycobacterium phlei CWS samples were found to contain muramic acid levels comparable to those found with a specific enzymatic assay. However, a colorimetric total hexosamine assay gave values 20-30% higher than those obtained by the specific PTC amino sugar assay. The detection limit of each PTC amino sugar was 5 pmol.

引用

页码：63 / 68

页数：6

共 14 条

[1] MASS-SPECTROMETRIC QUANTITATION OF MURAMIC ACID, A BACTERIAL-CELL WALL COMPONENT, IN SEPTIC SYNOVIAL-FLUIDS [J].