Genomic structure of the human NDUFS8 gene coding for the iron-sulfur TYKY subunit of the mitochondrial NADH:ubiquinone oxidoreductase

被引：18

作者：

de Sury, R ^{[1
]}

Martinez, P ^{[1
]}

Procaccio, V ^{[1
]}

Lunardi, J ^{[1
]}

Issartel, JP ^{[1
]}

机构：

[1] CEA, DBMS, Lab BECP EA 2019, F-38054 Grenoble 9, France

来源：

GENE | 1998年 / 215卷 / 01期

关键词：

mitochondrial respiratory chain; Complex I; nuclear gene; immunodetection; two-dimensional electrophoresis; chromosome; 11;

D O I：

10.1016/S0378-1119(98)00275-3

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The structural organization of the NDUFS8 gene coding for the TYKY subunit of the human mitochondrial NADH:ubiquinone oxidoreductase (Complex I) has been determined by sequencing of a genomic fragment cloned from a cosmid library. The NDUFS8 gene is located on chromosome 11q13 immediately downstream of the ALDH7 isoform gene. It spans about 6 kb and contains seven exons ranging in size from 51 to 186 bp. Three CCAAT box sequence motifs are present upstream of the transcription start. Spl and NRF1 binding site motifs are present in the first intron. Expression of the gene is ubiquitous but predominant in heart and skeletal muscle. Immunodetection of the TYKY subunit in placental mitochondria after two-dimensional gel electrophoresis revealed that the mature protein has a molecular mass of 22 kDa and a pi in the range of 4.9-5.0. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：1 / 10

页数：10

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