Genomic structure of the human NDUFS8 gene coding for the iron-sulfur TYKY subunit of the mitochondrial NADH:ubiquinone oxidoreductase

被引:18
作者
de Sury, R [1 ]
Martinez, P [1 ]
Procaccio, V [1 ]
Lunardi, J [1 ]
Issartel, JP [1 ]
机构
[1] CEA, DBMS, Lab BECP EA 2019, F-38054 Grenoble 9, France
关键词
mitochondrial respiratory chain; Complex I; nuclear gene; immunodetection; two-dimensional electrophoresis; chromosome; 11;
D O I
10.1016/S0378-1119(98)00275-3
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The structural organization of the NDUFS8 gene coding for the TYKY subunit of the human mitochondrial NADH:ubiquinone oxidoreductase (Complex I) has been determined by sequencing of a genomic fragment cloned from a cosmid library. The NDUFS8 gene is located on chromosome 11q13 immediately downstream of the ALDH7 isoform gene. It spans about 6 kb and contains seven exons ranging in size from 51 to 186 bp. Three CCAAT box sequence motifs are present upstream of the transcription start. Spl and NRF1 binding site motifs are present in the first intron. Expression of the gene is ubiquitous but predominant in heart and skeletal muscle. Immunodetection of the TYKY subunit in placental mitochondria after two-dimensional gel electrophoresis revealed that the mature protein has a molecular mass of 22 kDa and a pi in the range of 4.9-5.0. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
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页码:1 / 10
页数:10
相关论文
共 39 条
  • [1] Bovine-heart NADH:ubiquinone oxidoreductase is a monomer with 8 Fe-S clusters and 2 FMN groups
    Albracht, SPJ
    Mariette, A
    deJong, P
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1997, 1318 (1-2): : 92 - 106
  • [2] Ausenda C, 1996, Methods Enzymol, V264, P122, DOI 10.1016/S0076-6879(96)64013-0
  • [3] Prediction of complete gene structures in human genomic DNA
    Burge, C
    Karlin, S
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1997, 268 (01) : 78 - 94
  • [4] SELFISH DNA AND THE ORIGIN OF INTRONS
    CAVALIERSMITH, T
    [J]. NATURE, 1985, 315 (6017) : 283 - 284
  • [5] The NuoI subunit of the Rhodobacter capsulatus respiratory Complex I (equivalent to the bovine TYKY subunit) is required for proper assembly of the membraneous and peripheral domains of the enzyme
    Chevallet, M
    Dupuis, A
    Lunardi, J
    VanBelzen, R
    Albracht, SPJ
    Issartel, JP
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1997, 250 (02): : 451 - 458
  • [6] A nonradioactive double detection method for the assignment of spots in two-dimensional blots
    Chevallet, M
    Procaccio, V
    Rabilloud, T
    [J]. ANALYTICAL BIOCHEMISTRY, 1997, 251 (01) : 69 - 72
  • [7] Framework YAC contig anchored into a 3.2-Mb high-resolution physical map in proximal 11q13
    Courseaux, A
    Szepetowski, P
    Fernandes, M
    Serizet, C
    Kawaguchi, Y
    Grosgeorge, J
    PeruccaLostanlen, D
    Shows, TB
    Todd, JA
    Nowak, NJ
    Gaudray, P
    [J]. GENOMICS, 1997, 40 (01) : 13 - 23
  • [8] MOLECULAR-CLONING AND CHARACTERIZATION OF THE ACTIVE HUMAN MITOCHONDRIAL NADH-UBIQUINONE OXIDOREDUCTASE 24-KDA GENE (NDUFV2) AND ITS PSEUDOGENE
    DECOO, R
    BUDDIGER, P
    SMEETS, H
    VANKESSEL, AG
    MORGANHUGHES, J
    WEGHUIS, DO
    OVERHAUSER, J
    VANOOST, B
    [J]. GENOMICS, 1995, 26 (03) : 461 - 466
  • [9] Molecular cloning and characterization of the human mitochondrial NADH:oxidoreductase 10-kDa gene (NDUFV3)
    deCoo, RFM
    Buddiger, P
    Smeets, HJM
    vanOost, BA
    [J]. GENOMICS, 1997, 45 (02) : 434 - 437
  • [10] Primary structure of a ferredoxin-like iron-sulfur subunit of complex I from Neurospora crassa
    Duarte, M
    Finel, M
    Videira, A
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1996, 1275 (03): : 151 - 153