A nonradioactive double detection method for the assignment of spots in two-dimensional blots

被引：45

作者：

Chevallet, M

Procaccio, V

Rabilloud, T

机构：

[1] CEA-Lab. de Bioenergetique C., EA 2019, DBMS/BECP, F-38054 Grenoble Cedex 9

来源：

ANALYTICAL BIOCHEMISTRY | 1997年 / 251卷 / 01期

关键词：

D O I：

10.1006/abio.1997.2206

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A method for identification of spots on two-dimensional electrophoresis gels by means of immunoblotting is described. This method does not require radioactive-labeled proteins and is based on the dual use of colloidal gold staining to detect the general 2D pattern on the blot and on chemiluminescence to detect the antibody-reactive spot(s), Profit is taken from the fact that the general gold stain produces a background pattern on strong ECL exposures, which allows alignment of the antibody-reactive spot(s) on the general pattern and therefore easy identification of the spot(s) of interest on the two-dimensional maps. (C) 1997 Academic Press.

引用

收藏

页码：69 / 72

页数：4

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