Dysregulation of miRNA 181b in the temporal cortex in schizophrenia

被引：272

作者：

Beveridge, Natalie J. ^{[1
,2
,3
]}

Tooney, Paul A. ^{[1
,2
,3
]}

Carroll, Adam P. ^{[1
,2
,3
]}

Gardiner, Erin ^{[1
,2
,3
]}

Bowden, Nikola ^{[1
,2
,3
]}

Scott, Rodney J. ^{[1
,2
,3
]}

Tran, Nham ^{[5
]}

Dedova, Irina ^{[1
,4
]}

Cairns, Murray J. ^{[1
,2
,3
]}

机构：

[1] Schizophrenia Res Inst, Sydney, NSW 2006, Australia

[2] Univ Newcastle, Fac Hlth, Sch Biomed Sci, Callaghan, NSW 2308, Australia

[3] Univ Newcastle, Hunter Med Res Inst, Callaghan, NSW 2308, Australia

[4] Univ Sydney, Dept Pathol, Sydney, NSW 2006, Australia

[5] Univ Sydney, Dept Immunol & Infect Dis, Sydney, NSW 2006, Australia

来源：

HUMAN MOLECULAR GENETICS | 2008年 / 17卷 / 08期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1093/hmg/ddn005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Analysis of global microRNA (miRNA) expression in postmortem cortical grey matter from the superior temporal gyrus, revealed significant up-regulation of miR-181b expression in schizophrenia. This finding was supported by quantitative real-time RT-PCR analysis of miRNA expression in a cohort of 21 matched pairs of schizophrenia and non-psychiatric controls. The implications of this finding are substantial, as this miRNA is predicted to regulate many target genes with potential significance to the development of schizophrenia. They include the calcium sensor gene visinin-like 1 (VSNL1) and the ionotropic AMPA glutamate receptor subunit (GRIA2), which were found to be down-regulated in the same cortical tissue from the schizophrenia group. Both of these genes were also suppressed in miR-181b transfected cells and shown to contain functional miR-181b miRNA recognition elements by reporter gene assay. This study suggests altered miRNA levels could be a significant factor in the dysregulation of cortical gene expression in schizophrenia.

引用

页码：1156 / 1168

页数：13

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