Production and characterization of a silk-like hybrid protein, based on the polyalanine region of Samia cynthia ricini silk fibroin and a cell adhesive region derived from fibronectin

被引：54

作者：

Asakura, T ^{[1
]}

Tanaka, C

Yang, MY

Yao, JM

Kurokawa, M

机构：

[1] Tokyo Univ Agr & Technol, Dept Biotechnol, Koganei, Tokyo 1848588, Japan

[2] Sanyo Chem Ind Ltd, Higashiyama Ku, Kyoto 6050995, Japan

来源：

BIOMATERIALS | 2004年 / 25卷 / 04期

关键词：

silk; fibronectin; RGD peptide; genetic engineering; cell adhesion; cell culture;

D O I：

10.1016/S0142-9612(03)00570-2

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

There are a variety of silkworms and silk fibroins produced by them. Silks have many inherent suitable properties for biomaterials. In this paper, a novel silk-like hybrid protein, [DGG(A)(12)GGAASTGRGDSPAAS](5), which consists of polyalanine region of silk fibroin from a wild silkworm, Samia cynthia ricini, and cell adhesive region including Arg-Gly-Asp (RGD) sequence, derived from fibronectin, was designed and produced. The genes encoding the hybrid protein were constructed and expressed in Escherichia coli. The main conformation of the polyalanine region, that is, either alpha-helix or beta-sheet, could be easily controlled by treatment with different acidic solvents, trifluoroacetic acid or formic acid, respectively. This structural change was monitored with C-13 CP/MAS NMR. Higher cell adhesive and growth activities of the hybrid protein compared with those of collagen were obtained. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：617 / 624

页数：8

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