Improvement of biodiesel production by lipozyme TL IM-catalyzed methanolysis using response surface methodology and acyl migration enhancer

被引：102

作者：

Wang, Y. ^{[1
]}

Wu, H. ^{[1
]}

Zong, M. H. ^{[1
]}

机构：

[1] S China Univ Technol, Lab Appl Biocatalysis, Guangzhou 510640, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2008年 / 99卷 / 15期

关键词：

biodiesel; lipozyme TL IM; RSM; triethylamine; acyl migration;

D O I：

10.1016/j.biortech.2007.12.062

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The process of biodiesel production from corn oil catalyzed by lipozyme TL IM, an inexpensive 1,3-position specific lipase from Thermomyces lanuginosus was optimized by response surface methodology (RSM) and a central composite rotatable design (CCRD) was used to study the effects of enzyme dosage, ratio of t-butanol to oil (v/v) and ratio of methanol to oil (mol/mol) on the methyl esters (ME) yield of the methanolysis. The optimum combinations for the reaction were 25.9 U/g(oil) of enzyme, 0.58 volume ratio of t-butanol to oil and 0.5, 0.5, 2.8 molar equivalent of methanol to oil added at the reaction time of 0, 2, and 4 h, respectively, by which a ME yield of 85.6%, which was very close to the predicted value of 85.0%, could be obtained after reaction for 12 h. Waste oil was found to be more suitable feedstock, and could give 93.7% ME yield under the optimum conditions described above. Adding triethylamine (TEA), an acyl migration enhancer, could efficiently improve the ME yield of the methanolysis of corn oil, giving a ME yield of 92.0%. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：7232 / 7237

页数：6

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