Validation and comparison of luminex multiplex cytokine analysis kits with ELISA: Determinations of a panel of nine cytokines in clinical sample culture supernatants

被引:239
作者
duPont, NC
Wang, KH
Wadhwa, PD
Culhane, JF
Nelson, EL
机构
[1] Univ Calif Irvine, Dept Obstet & Gynecol, Div Gynecol Oncol, Orange, CA 92868 USA
[2] Univ Calif Irvine, Dept Med, Div Hematol Oncol, Irvine, CA 92697 USA
[3] Univ Calif Irvine, Dept Psychiat & Human Behav, Irvine, CA 92697 USA
[4] Drew Univ, Coll Med, Sch Publ Hlth, Dept Obstet & Gynecol, Philadelphia, PA USA
[5] Univ Calif Irvine, Sch Biol Sci, Dept Mol Biol & Biochem, Irvine, CA 92697 USA
关键词
reproductive immunology; clinical study; cytokine; ELISA; luminex; multiplex analysis;
D O I
10.1016/j.jri.2005.03.005
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Problem: Analyses of the expression pattern of multiple cytokines are frequently required for characterization of the status of the immune system as it pertains to Th type bias and intrinsic levels of inflammation. Classically, analysis of cytokine expression patterns has been performed by enzyme-linked immunosorbent assays (ELISA) for each separate analyte. A new technology, Luminex MAP(R), facilitates the simultaneous evaluation of multiple immune mediators with advantages of higher throughput, smaller sample volume, and lower cost. Validation of this technology has been limited to small sample sets, limited use of clinical study specimens, and use of non-commercial reagents. Methods: Ninety-six specimens from women over the course of their respective pregnancies were evaluated for cytokine concentrations using commercially available ELISA kits and commercially available Luminex MAP(R) kits according to the manufacturers' directions. Correlations between data sets were evaluated using Pearson's correlation coefficient (r). Results: Excellent correlations were demonstrated for IL-1 beta, IL-4, IL-5, IL-6, IL-10, IFN gamma, and TNF alpha, in contrast to IL-12 p70 and IL-13. Conclusions: Luminex multiplex technology has distinct advantages and is a valid alternative method to ELISA for the evaluation of the majority of cytokines tested and for the characterization of immune system status. (C) 2005 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:175 / 191
页数:17
相关论文
共 77 条
  • [21] Stress hormones, proinflammatory and antiinflammatory cytokines, and autoimmunity
    Elenkov, IJ
    Chrousos, GP
    [J]. NEUROENDOCRINE IMMUNE BASIS OF THE RHEUMATIC DISEASES II, PROCEEDINGS, 2002, 966 : 290 - 303
  • [22] TRANSCRIPTIONAL AND POSTTRANSCRIPTIONAL REGULATION OF INTERLEUKIN-1 GENE-EXPRESSION - REVIEW
    FENTON, MJ
    [J]. INTERNATIONAL JOURNAL OF IMMUNOPHARMACOLOGY, 1992, 14 (03): : 401 - 411
  • [23] Fulton RJ, 1997, CLIN CHEM, V43, P1749
  • [24] PROCEDURES FOR DETECTING OUTLYING OBSERVATIONS IN SAMPLES
    GRUBBS, FE
    [J]. TECHNOMETRICS, 1969, 11 (01) : 1 - &
  • [25] Hildesheim A, 2002, CANCER EPIDEM BIOMAR, V11, P1477
  • [26] Hoffmann E, 2002, J LEUKOCYTE BIOL, V72, P847
  • [27] Hutchings Anne, 2003, Human Immunology, V64, pS10, DOI 10.1016/j.humimm.2003.08.014
  • [28] Hutchinson KL, 2001, J MED VIROL, V65, P561, DOI 10.1002/jmv.2073
  • [29] Jenison R, 2001, CLIN CHEM, V47, P1894
  • [30] Cytokines as mediators for or effectors against rotavirus disease in children
    Jiang, B
    Snipes-Magaldi, L
    Dennehy, P
    Keyserling, H
    Holman, RC
    Bresee, J
    Gentsch, J
    Glass, RI
    [J]. CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 2003, 10 (06) : 995 - 1001