Epigenetic factors influencing resistance to nuclear reprogramming

被引：74

作者：

Pasque, Vincent ^{[1
,2
]}

Jullien, Jerome ^{[1
,2
]}

Miyamoto, Kei ^{[1
,2
]}

Halley-Stott, Richard P. ^{[1
,2
]}

Gurdon, J. B. ^{[1
,2
]}

机构：

[1] Univ Cambridge, Wellcome Trust Canc Res UK Gurdon Inst, Cambridge CB2 1QN, England

[2] Univ Cambridge, Dept Zool, Cambridge CB2 3EJ, England

来源：

TRENDS IN GENETICS | 2011年 / 27卷 / 12期

基金：

日本学术振兴会; 新加坡国家研究基金会; 英国惠康基金;

关键词：

PLURIPOTENT STEM-CELLS; HISTONE VARIANT MACROH2A; SMALL-MOLECULE COMPOUNDS; HUMAN SOMATIC-CELLS; XENOPUS-LAEVIS; DNA DEMETHYLATION; LINKER HISTONE; DIFFERENTIATED CELLS; GENE-EXPRESSION; DEFINED FACTORS;

D O I：

10.1016/j.tig.2011.08.002

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Patient-specific somatic cell reprogramming is likely to have a large impact on medicine by providing a source of cells for disease modelling and regenerative medicine. Several strategies can be used to reprogram cells, yet they are generally characterised by a low reprogramming efficiency, reflecting the remarkable stability of the differentiated state. Transcription factors, chromatin modifications, and noncoding RNAs can increase the efficiency of reprogramming. However, the success of nuclear reprogramming is limited by epigenetic mechanisms that stabilise the state of gene expression in somatic cells and thereby resist efficient reprogramming. We review here the factors that influence reprogramming efficiency, especially those that restrict the natural reprogramming mechanisms of eggs and oocytes. We see this as a step towards understanding the mechanisms by which nuclear reprogramming takes place.

引用

页码：516 / 525

页数：10

共 103 条

[1] The histone variant H3.3 marks active chromatin by replication-independent nucleosome assembly [J].