Precise excision and transposition of piggyBac in pink bollworm embryos

被引：42

作者：

Thibault, ST ^{[1
]}

Luu, HT ^{[1
]}

Vann, N ^{[1
]}

Miller, TA ^{[1
]}

机构：

[1] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA

来源：

INSECT MOLECULAR BIOLOGY | 1999年 / 8卷 / 01期

关键词：

piggyBac; pink bollworm; transposable element; transformation;

D O I：

10.1046/j.1365-2583.1999.810119.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transposable elements such as P, hobo, Hermes, mariner and Mines have been successfully harnessed as gene vectors to achieve the transformation of several dipteran species including Drosophila melanogaster, Ceratitis capitata and Aedes aegypti, Plasmid-based excision and transposition assays have been useful indicators of an element's ability to be mobilized in vivo and thus potentially serve as a transforming vector, We report that the transposable element piggyBac is capable of precise excision and transposition in the pink bollworm (Pectinophora gossypiella), a worldwide pest of cultivated cotton. Combined with a suitable marker gene, the piggyBac element may serve as a vector for germline transformation in this and (potentially) other lepidopteran species.

引用

页码：119 / 123

页数：5

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