MEMS devices for detecting correspondence between mechanical rotation and ATP consumption in a single biomolecular motor

被引：6

作者：

Arata, H ^{[1
]}

Takeuchi, S ^{[1
]}

Tresset, G ^{[1
]}

Rondelez, Y ^{[1
]}

Tabata, K ^{[1
]}

Noji, H ^{[1
]}

Fujita, H ^{[1
]}

机构：

[1] Univ Tokyo, Inst Ind Sci, CIRMM, Tokyo, Japan

来源：

MEMS 2004: 17TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS, TECHNICAL DIGEST | 2004年

关键词：

D O I：

10.1109/MEMS.2004.1290597

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

The device for controlling rotational velocity and measuring ATP consumption, simultaneously, of a single F-1-ATPase, a rotary biomolecular motor, was developed. This device is equipped with the temperature control device on a slide glass and PDMS micro chamber which volume is on the order of 10 femtoliters. The rotation velocity of a single F-1 is controlled by changing the temperature within short response time, 2 seconds. The size of the micro chamber is so small that concentration change of products of a single molecular enzyme can be measured in tens of minutes. To show the feasibility of micro chamber in measuring ATP consumption of a single F-1, the catalytic activity measurement of a single HRP enzyme was demonstrated.

引用

页码：363 / 366

页数：4

共 6 条

[1]

ARATA H, 2003, P 20 SENS S SENS MIC, P119

[2] Direct observation of the rotation of ε subunit in F1-ATPase [J].