Heavy metal tolerance of transgenic tobacco plants over-expressing cysteine synthase

被引：105

作者：

Kawashima, CG

Noji, M

Nakamura, M

Ogra, Y

Suzuki, KT

Saito, K

机构：

[1] Chiba Univ, Grad Sch Pharmaceut Sci, Dept Mol Biol & Biotechnol, Inage Ku, Chiba 2638522, Japan

[2] Chiba Univ, Grad Sch Pharmaceut Sci, Dept Toxicol & Environm Hlth, Inage Ku, Chiba 2638522, Japan

来源：

BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 02期

基金：

日本科学技术振兴机构;

关键词：

cysteine biosynthesis; heavy metal tolerance; phytochelatin; phytoremediation; transgenic plants;

D O I：

10.1023/B:BILE.0000012895.60773.ff

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cysteine synthase [O-acetyl-L-serine(thiol) lyase] catalyzes the final step for L-cysteine biosynthesis in plants. The tolerance of transgenic tobacco plants over-expressing cysteine synthase cDNA in cytosol (3F), chloroplasts (4F) and in both organelles (F-1) was investigated towards heavy metals such as Cd, Se, Ni, Pb and Cu. The transgenic plants were significantly more tolerant than wild-type plants in agar medium containing Cd, Se and Ni. The F-1 transgenic plants had a higher resistance than other transgenic lines towards these metals and could enhance accumulation of Cd in shoot. These results suggest that the transgenic plants over-expressing cysteine synthase both in cytosol and chloroplasts can be applicable to phyto-remediation of Cd from contaminated soils.

引用

页码：153 / 157

页数：5

共 14 条

[1] Detoxification of cadmium in tobacco plants: formation and active excretion of crystals containing cadmium and calcium through trichomes [J].