Preparation and characterization of chitosan-hydroxyapatite-glycopolymer/Cloisite 30 B nanocomposite for biomedical applications

A new composite was synthesized from chitosan/hydroxyapatite and glycopolymer grafted onto a Cloisite 30B clay surface (CS/HAP/clay-Gh) in aqueous media via a solvent casting and evaporation method. Another composite containing chitosan/hydroxyapatite and pristine Cloisite 30B (CS/HAP/clay) was prepared and compared with the (CS/HAP/clay-Gh). The resulting composites were characterized using various analytical tools such as Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermal gravimetric analyses (TGA) and scanning electron microscopy (SEM). Water uptake capability or swelling was tested for each composite. The cytotoxicity of the resulting tri-component composites was tested against a breast carcinoma cell line (MCF-7), liver carcinoma cell line (HepG-2) and a normal human skin fibroblast cell line (BJ-1) by MTT [3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide] as well as LDH (lactate dehydrogenase) assay. The obtained preliminary results showed good properties for CS/HAP/clay-Gh on the way to be used in the future in the biomedical applications.