Age-dependent changes in CXC chemokine ligand 10 serum levels in euthyroid subjects

Circulating levels of cytokines are deeply influenced by aging, and few data about serum chemokines are available. The aim of this study was to evaluate the influence of aging on circulating CXCL10. One hundred forty healthy subjects ( 70 males and 70 females), 10 - 79 years of age, underwent fasting plasma glucose, total cholesterol, high- density lipoprotein ( HDL), low- density lipoprotein ( LDL), triglyceride, and CXCL8 serum assay. Thyroid hormone testing for thyroid- stimulating hormone ( TSH), antithyroglobulin ( AbTg), and anti-thyroperoxidase ( AbTPO) autoantibodies and thyroid ultrasonography were performed in all subjects to exclude the presence of clinical or subclinical thyroid disease. Serum CXCL10 levels were assayed in all subjects and found to be increased in 14 of 70 females ( 20%) and in 4 of 70 males ( 5.7%) ( p = 0.01). In a multiple linear regression model including age, body mass index ( BMI), systolic and diastolic blood pressure, glycemia, total cholesterol, HDL, LDL, triglycerides, TSH, AbTPO, AbTg, and CXCL8, only age was significantly related to CXCL10 [ C. R. 1.30 ( 0.28- 2.33), p = 0.001]. No relationship was present between CXCL8 serum levels and age, suggesting a specificity of CXCL10 elevation as a function of age. Results of this study, performed in healthy subjects on an age gradient, demonstrate an increase in serum CXCL10 with advancing age overall in females, supporting the hypothesis of enhanced Th1 immune responses in aging.