A single residue mutation of 5-enoylpyruvylshikimate-3-phosphate synthase in Pseudomonas stutzeri enhances resistance to the herbicide glyphosate

被引：34

作者：

Liang, Aimin ^{[1
,2
]}

Sha, Jiying ^{[1
,3
]}

Lu, Wei ^{[1
]}

Chen, Ming ^{[1
]}

Li, Liang ^{[1
]}

Jin, Dan ^{[4
]}

Yan, Yongliang ^{[1
]}

Wang, Jin ^{[1
,5
]}

Ping, Shuzhen ^{[1
]}

Zhang, Wei ^{[1
]}

Wang, Yiding ^{[3
]}

Lin, Min ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China

[2] China Agr Univ, Coll Biol Sci, Beijing 100094, Peoples R China

[3] Sichuan Normal Univ, Coll Life Sci, Chengdu 610066, Peoples R China

[4] SW Univ, Biotechnol Res Ctr, Chongqing 400716, Peoples R China

[5] Mianyang Normal Coll, Plant Genom Res Ctr, Mianyang 621000, Sichuan, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 08期

关键词：

aroA gene; 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS); error-prone PCR; glyphosate; Pseudomonas stutzeri;

D O I：

10.1007/s10529-008-9703-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel class II 5-enoylpyruvylshikimate-3-phosphate synthase (EPSPS) was identified from Pseudomonas stutzeri A1501 by complementation of an Escherichia coli auxotrophic aroA mutant. The single amino acid substitution of serine (Ser) for asparagine (Asn)-130 of the A1501 EPSPS enhanced resistance to 200 mM glyphosate. The mutated EPSPS had a 2.5-fold increase for IC50 [glyphosate] value, a 2-fold increase for K-i [glyphosate] value, but a K-m [PEP] value similar to that of wild type. The effect of the single residue mutation on glyphosate resistance was also analyzed using a computer-based three-dimensional model.

引用

页码：1397 / 1401

页数：5

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