Fermentable-sugar-level-dependent regulation of leukotoxin synthesis in a variably toxic strain of Actinobacillus actinomycetemcomitans

被引:21
作者
Inoue, T
Tanimoto, I
Tada, T
Ohashi, T
Fukui, K
Ohta, H
机构
[1] Okayama Univ, Sch Dent, Dept Microbiol, Okayama 7008525, Japan
[2] Okayama Univ, Sch Dent, Dept Prevent Dent, Okayama 7008525, Japan
[3] Okayama Univ, Sch Dent, Dept Periodontol & Endodontol, Okayama 7008525, Japan
[4] Ibaraki Univ, Sch Agr, Dept Bioresource Sci, Microbial Ecol Lab, Ami, Ibaraki 3000393, Japan
来源
MICROBIOLOGY-SGM | 2001年 / 147卷
关键词
RTX toxin; periodontopathogen; chemostat culture; catabolite repression;
D O I
10.1099/00221287-147-10-2749
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Actinobacillus actinomycetemcomitans, a Gram-negative periodontopathic bacterium, produces a leukotoxin belonging to the RTX family. The production of leukotoxin varies greatly among different strains of this species and under different culture conditions. A toxin-production-variable strain, 301-b, stably produces significant amounts of leukotoxin in anaerobic fructose-limited chemostat cultures, but does not do so in the presence of excess fructose. This communication describes the cloning and sequencing of the leukotoxin promoter region from 301-b, showing that this strain has a promoter region similar to that from strain 652, a moderately toxic strain. Northern blot analysis using a leukotoxin gene probe demonstrated that change in toxin production in response to the level of external fructose was due to alteration in the transcriptional level of the leukotoxin gene. Pulsing of fructose into the fructose-limited chemostat culture remarkably reduced the intracellular cAMP level from 40 pmol (mg dry wt cells)(-1) to 3.1 pmol (mg dry wt cells)(-1), which was restored when the culture was returned to fructose-limited conditions. Further, it was found that addition of external cAMP to the culture with excess fructose resulted in an apparent recovery of leukotoxin production. Taken together, these findings indicate that a cAMP-dependent mechanism, possibly a catabolite-repression-like system, may be involved in the regulation of leukotoxin production in this bacterium.
引用
收藏
页码:2749 / 2756
页数:8
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