HIV-1 gp160 decreases the K+ voltage-gated current from Jurkat E6.1 T cells by up-phosphorylation

被引：14

作者：

Dellis, O

Bouteau, F

Guenounou, M

Rona, JP

机构：

[1] Univ Paris 07, LPCMSP, Lab Electrophysiol Membranes, F-75251 Paris 05, France

[2] Univ Reims, Fac Pharm, Lab Immunol & Virol Appl, F-51110 Reims 01, France

来源：

FEBS LETTERS | 1999年 / 443卷 / 02期

关键词：

human T cell; potassium channel; HIV-1; gp160; protein kinase C; membrane potential; p56(lck);

D O I：

10.1016/S0014-5793(98)01691-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

HIV-1 gp120/gp160 is known to disturb the activity of p56(lck), protein kinase C (PKC) and Ca2+ homeostasis in T lymphocytes. We found that gp160 decreases the Kv1.3 current of Jurkat E6.1 cells probably by increasing the PKC-dependent phosphorylation of Kv channel protein after 5 days. This decrease is dose-dependent. In contrast, gp160 did not decrease the Kv1.3 current of the JCaM1.6 cell line, a p56(lck)-defective Jurkat cell line. This shows that p56(lck) was at the beginning of the events which induced the Kv1.3 current decrease. As a consequence of this decrease, Jurkat E6.1 cells were depolarized and exhibited a volume increase. (C) 1999 Federation of European Biochemical Societies.

引用

页码：187 / 191

页数：5

共 42 条

[1] PROGRAMMED CELL-DEATH AND AIDS - FROM HYPOTHESIS TO EXPERIMENT [J].