Real-time reverse transcription-polymerase chain reaction assay for SARS-associated coronavirus

被引：224

作者：

Emery, SL ^{[1
]}

Erdman, DD ^{[1
]}

Bowen, MD ^{[1
]}

Newton, BR ^{[1
]}

Winchell, JM ^{[1
]}

Meyer, RF ^{[1
]}

Tong, SX ^{[1
]}

Cook, BT ^{[1
]}

Holloway, BP ^{[1
]}

McCaustland, KA ^{[1
]}

Rota, PA ^{[1
]}

Bankamp, B ^{[1
]}

Lowe, LE ^{[1
]}

Ksiazek, TG ^{[1
]}

Bellini, WJ ^{[1
]}

Anderson, LJ ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, Atlanta, GA 30333 USA

来源：

EMERGING INFECTIOUS DISEASES | 2004年 / 10卷 / 02期

关键词：

D O I：

10.3201/eid1002.030759

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of <10 genomic copies per reaction. The real-time RTPCR assay was more sensitive than a conventional RTPCR assay or culture isolation and proved suitable to detect SARS-CoV in clinical specimens. Application of this assay will aid in diagnosing SARS-CoV infection.

引用

页码：311 / 316

页数：6

共 16 条

[1]

CJERMESLU MA, 1996, VIROLOGY METHODS MAN, P107

[2] Identification of a novel coronavirus in patients with severe acute respiratory syndrome [J].

Drosten, C ;

Günther, S ;

Preiser, W ;

van der Werf, S ;

Brodt, HR ;

Becker, S ;

Rabenau, H ;

Panning, M ;

Kolesnikova, L ;

Fouchier, RAM ;

Berger, A ;

Burguière, AM ;

Cinatl, J ;

Eickmann, M ;

Escriou, N ;

Grywna, K ;

Kramme, S ;

Manuguerra, JC ;

Müller, S ;

Rickerts, V ;

Stürmer, M ;

Vieth, S ;

Klenk, HD ;

Osterhaus, ADME ;

Schmitz, H ;

Doerr, HW .

NEW ENGLAND JOURNAL OF MEDICINE, 2003, 348 (20) :1967-1976

[3] A novel coronavirus associated with severe acute respiratory syndrome [J].

Ksiazek, TG ;

Erdman, D ;

Goldsmith, CS ;

Zaki, SR ;

Peret, T ;

Emery, S ;

Tong, SX ;

Urbani, C ;

Comer, JA ;

Lim, W ;

Rollin, PE ;

Dowell, SF ;

Ling, AE ;

Humphrey, CD ;

Shieh, WJ ;

Guarner, J ;

Paddock, CD ;

Rota, P ;

Fields, B ;

DeRisi, J ;

Yang, JY ;

Cox, N ;

Hughes, JM ;

LeDuc, JW ;

Bellini, WJ ;

Anderson, LJ .

NEW ENGLAND JOURNAL OF MEDICINE, 2003, 348 (20) :1953-1966

[4] Rapid detection of West Nile virus from human clinical specimens, field-collected mosquitoes, and avian samples by a TaqMan reverse transcriptase-PCR assay [J].

Lanciotti, RS ;

Kerst, AJ ;

Nasci, RS ;

Godsey, MS ;

Mitchell, CJ ;

Savage, HM ;

Komar, N ;

Panella, NA ;

Allen, BC ;

Volpe, KE ;

Davis, BS ;

Roehrig, JT .

JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (11) :4066-4071

[5] A major outbreak of severe acute respiratory syndrome in Hong Kong [J].

Lee, N ;

Hui, D ;

Wu, A ;

Chan, P ;

Cameron, P ;

Joynt, GM ;

Ahuja, A ;

Yung, MY ;

Leung, CB ;

To, KF ;

Lui, SF ;

Szeto, CC ;

Chung, S ;

Sung, JJY .

NEW ENGLAND JOURNAL OF MEDICINE, 2003, 348 (20) :1986-1994

[6] Real-time PCR in virology [J].

Mackay, IM ;

Arden, KE ;

Nitsche, A .

NUCLEIC ACIDS RESEARCH, 2002, 30 (06) :1292-1305

[7] Clinical progression and viral load in a community outbreak of coronavirus-associated SARS pneumonia: a prospective study [J].

Peiris, JSM ;

Chu, CM ;

Cheng, VCC ;

Chan, KS ;

Hung, IFN ;

Poon, LLM ;

Law, KI ;

Tang, BSF ;

Hon, TYW ;

Chan, CS ;

Chan, KH ;

Ng, JSC ;

Zheng, BJ ;

Ng, WL ;

Lai, RWM ;

Guan, Y ;

Yuen, KY .

LANCET, 2003, 361 (9371) :1767-1772

[8] Rapid diagnosis of a coronavirus associated with severe acute respiratory syndrome (SARS) [J].

Poon, LLM ;

Wong, OK ;

Luk, W ;

Yuen, KY ;

Peiris, JSM ;

Guan, Y .

CLINICAL CHEMISTRY, 2003, 49 (06) :953-955

[9] Identification of severe acute respiratory syndrome in Canada [J].

Poutanen, SM ;

Low, DE ;

Henry, B ;

Finkelstein, S ;

Rose, D ;

Green, K ;

Tellier, R ;

Draker, R ;

Adachi, D ;

Ayers, M ;

Chan, AK ;

Skowronski, DM ;

Salit, I ;

Simor, AE ;

Slutsky, AS ;

Doyle, PW ;

Krajden, M ;

Petric, M ;

Brunham, RC ;

McGeer, AJ .

NEW ENGLAND JOURNAL OF MEDICINE, 2003, 348 (20) :1995-2005

[10] Characterization of a novel coronavirus associated with severe acute respiratory syndrome [J].

Rota, PA ;

Oberste, MS ;

Monroe, SS ;

Nix, WA ;

Campagnoli, R ;

Icenogle, JP ;

Peñaranda, S ;

Bankamp, B ;

Maher, K ;

Chen, MH ;

Tong, SX ;

Tamin, A ;

Lowe, L ;

Frace, M ;

DeRisi, JL ;

Chen, Q ;

Wang, D ;

Erdman, DD ;

Peret, TCT ;

Burns, C ;

Ksiazek, TG ;

Rollin, PE ;

Sanchez, A ;

Liffick, S ;

Holloway, B ;

Limor, J ;

McCaustland, K ;

Olsen-Rasmussen, M ;

Fouchier, R ;

Günther, S ;

Osterhaus, ADME ;

Drosten, C ;

Pallansch, MA ;

Anderson, LJ ;

Bellini, WJ .

SCIENCE, 2003, 300 (5624) :1394-1399

← 1 2 →