The internalization pathway, metabolic fate and biological effect of superparamagnetic iron oxide nanoparticles in the macrophage-like RAW264.7 cell

被引：103

作者：

Gu JingLi ^{[1
]}

Xu HaiFei ^{[1
]}

Han YeHua ^{[2
]}

Dai Wei ^{[2
]}

Hao Wei ^{[1
]}

Wang ChunYu ^{[3
]}

Gu Ning ^{[3
]}

Xu HaiYan ^{[4
]}

Cao JiMin ^{[1
]}

机构：

[1] Chinese Acad Med Sci, Peking Union Med Coll, Inst Basic Med Sci, Sch Basic Med,Dept Physiol & Pathophysiol, Beijing 100005, Peoples R China

[2] Chinese Acad Med Sci, Peking Union Med Coll, Inst Basic Med Sci, Sch Basic Med,Ctr Electromicroscope, Beijing 100005, Peoples R China

[3] Southeast Univ, Sch Biol Sci & Med Engn, Jiangsu Lab Biomat & Devices, State Key Lab Bioelect, Nanjing 211189, Peoples R China

[4] Chinese Acad Med Sci, Peking Union Med Coll, Inst Basic Med Sci, Sch Basic Med,Dept Biomed Engn, Beijing 100005, Peoples R China

来源：

SCIENCE CHINA-LIFE SCIENCES | 2011年 / 54卷 / 09期

基金：

中国国家自然科学基金; 国家高技术研究发展计划(863计划);

关键词：

iron oxide nanoparticles; contrast medium; macrophage; endocytosis; iron metabolism; CLATHRIN-MEDIATED ENDOCYTOSIS; CARBON NANOTUBES; CONTRAST AGENT; MOLECULAR CONTROL; ACTIN DYNAMICS; FERRITIN; EXOCYTOSIS; TRACKING; CHOLESTEROL; FERUMOXIDES;

D O I：

10.1007/s11427-011-4215-5

中图分类号：

Q [生物科学];

学科分类号：

090105 [作物生产系统与生态工程];

摘要：

The potential applications of superparamagnetic iron oxide nanoparticles (SPIONs) in several nanomedical fields have attracted intense interest based on the cell-nano interaction. However, the mechanisms underlying cell uptake, the intracellular trail, final fate and the biological effects of SPIONs have not yet been clearly elucidated. Here, we showed that multiple endocytic pathways were involved in the internalization process of SPIONs in the RAW264.7 macrophage. The internalized SPIONs were biocompatible and used three different metabolic pathways: The SPIONs were distributed to daughter cells during mitosis; they were degraded in the lysosome and free iron was released into the intracellular iron metabolic pool; and, the intact SPIONs were potentially exocytosed out of the cells. The internalized SPIONs did not induce cell damage but affected iron metabolism, inducing the upregulation of ferritin light chain at both the mRNA and protein levels and ferroportin 1 at the mRNA level. These results may contribute to the development of nanobiology and to the safe use of SPIONs in medicine when administered as a contrast medium or a drug delivery tool.

引用

页码：793 / 805

页数：13

共 52 条

[1]

Characterization of biophysical and metabolic properties of cells labeled with superparamagnetic iron oxide nanoparticles and transfection agent for cellular MR imaging [J].