Improved low-energy electron injection systems for high rate electron capture dissociation in Fourier transform ion cyclotron resonance mass spectrometry

被引：105

作者：

Tsybin, YO

Håkansson, P

Budnik, BA

Haselmann, KF

Kjeldsen, F

Gorshkov, M

Zubarev, RA

机构：

[1] Univ So Denmark, Dept Chem, DK-5230 Odense M, Denmark

[2] Uppsala Univ, Angstrom Lab, Ion Phys Div, SE-75121 Uppsala, Sweden

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2001年 / 15卷 / 19期

关键词：

D O I：

10.1002/rcm.448

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

New low-energy electron injection systems based on indirectly heated dispenser cathodes facilitate electron capture dissociation (ECD) in Fourier transform ion cyclotron resonance (FTICR) mass spectrometry. In this joint report, details are presented of the design and performance of these systems on two commercial FTICR instruments, 9.4 T Bruker BioAPEX in Uppsala and 4.7 T IonSpec Ultima in Odense. New results include obtaining meaningful one-scan MS/MS data for isolated precursor ions with millisecond irradiation times. The ECD rate improvement is not only due to the larger total electron current, but the larger emitting area as well. Copyright (C) 2001 John Wiley & Sons, Ltd.

引用

页码：1849 / 1854

页数：6

共 23 条

[21] Electron capture dissociation of gaseous multiply-charged proteins is favored at disulfide bonds and other sites of high hydrogen atom affinity [J].