Raji B cells, misidentified as THP-1 cells, stimulate DC-SIGN-mediated HIV transmission

被引：121

作者：

Wu, L

Martin, TD

Carrington, M

KewalRamani, VN ^{[1
]}

机构：

[1] NCI, Model Dev Sect, HIV Drug Resistance Program, NIH, Frederick, MD 21702 USA

[2] NCI, Basic Res Program, SAIC Frederick Inc, NIH, Frederick, MD 21702 USA

来源：

VIROLOGY | 2004年 / 318卷 / 01期

关键词：

HIV-1; DC-SIGN; dendritic cells; Raji cells; THP-1; cells; transmission;

D O I：

10.1016/j.virol.2003.09.028

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A number of studies examining interactions of dendritic cell (DC)-specific ICAM-3 grabbing nonintegrin (DC-SIGN) with viral pathogens have relied on monocytic transfectants as models for primary DCs. Here we show that the presumed "THP-1" monocytic cells used in these studies are instead Raji B cells. Moreover, we demonstrate that true THP-1 cells do not support DC-SIGN-mediated HIV-1 transmission, whereas human B cell lines efficiently enhance this process. These data indicate that there are features common to B cells and DCs that facilitate transmission of HIV-1 and provide new insights toward the mechanism of DC-SIGN-mediated HIV-1 transmission. Published by Elsevier Inc.

引用

收藏

页码：17 / 23

页数：7

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