Protein engineering by cell-surface display

被引：145

作者：

Wittrup, KD ^{[1
]}

机构：

[1] MIT, Dept Chem Engn, Cambridge, MA 02139 USA

[2] MIT, Dept Bioengn, Cambridge, MA 02139 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2001年 / 12卷 / 04期

关键词：

D O I：

10.1016/S0958-1669(00)00233-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein libraries displayed on cell surfaces can be labeled with soluble ligands exhibiting well-characterized binding equilibria and dissociation kinetics, and then quantitatively screened by flow cytometry at a rate of > 10(4) clones/second. The promise of cell-surface display for directed evolution is being realized, with significant improvements recently reported in protein ligand binding affinity, stability, expression and enzymatic activity.

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收藏

页码：395 / 399

页数：5

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