Protein engineering by cell-surface display

被引：145

作者：

Wittrup, KD ^{[1
]}

机构：

[1] MIT, Dept Chem Engn, Cambridge, MA 02139 USA

[2] MIT, Dept Bioengn, Cambridge, MA 02139 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2001年 / 12卷 / 04期

关键词：

D O I：

10.1016/S0958-1669(00)00233-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein libraries displayed on cell surfaces can be labeled with soluble ligands exhibiting well-characterized binding equilibria and dissociation kinetics, and then quantitatively screened by flow cytometry at a rate of > 10(4) clones/second. The promise of cell-surface display for directed evolution is being realized, with significant improvements recently reported in protein ligand binding affinity, stability, expression and enzymatic activity.

引用

收藏

页码：395 / 399

页数：5

相关论文

共 60 条

[51] Biosynthetic polypeptide libraries [J].

Shusta, EV ;

VanAntwerp, J ;

Wittrup, KD .

CURRENT OPINION IN BIOTECHNOLOGY, 1999, 10 (02) :117-122

[52] Directed evolution of a stable scaffold for T-cell receptor engineering [J].

Shusta, EV ;

Holler, PD ;

Kieke, MC ;

Kranz, DM ;

Wittrup, KD .

NATURE BIOTECHNOLOGY, 2000, 18 (07) :754-759

[53] Small binding proteins selected from a combinatorial repertoire of knottins displayed on phage [J].

Smith, GP ;

Patel, SU ;

Windass, JD ;

Thornton, JM ;

Winter, G ;

Griffiths, AD .

JOURNAL OF MOLECULAR BIOLOGY, 1998, 277 (02) :317-332

[54] FILAMENTOUS FUSION PHAGE - NOVEL EXPRESSION VECTORS THAT DISPLAY CLONED ANTIGENS ON THE VIRION SURFACE [J].

SMITH, GP .

SCIENCE, 1985, 228 (4705) :1315-1317

[55] Engineering a mouse metallothionein on the cell surface of Ralstonia eutropha CH34 for immobilization of heavy metals in soil [J].

Valls, M ;

Atrian, S ;

de Lorenzo, V ;

Fernández, LA .

NATURE BIOTECHNOLOGY, 2000, 18 (06) :661-665

[56] Fine affinity discrimination by yeast surface display and flow cytometry [J].

VanAntwerp, JJ ;

Wittrup, KD .

BIOTECHNOLOGY PROGRESS, 2000, 16 (01) :31-37

[57]

Westerlund-Wikström B, 2000, INT J MED MICROBIOL, V290, P223

[58] Surface expression of CD28 single chain Fv for costimulation by tumor cells [J].

Winberg, G ;

Grosmaire, LS ;

Klussman, K ;

Hayden, MS ;

Fell, HP ;

Ledbetter, JA ;

Mittler, RS .

IMMUNOLOGICAL REVIEWS, 1996, 153 :209-223

[59]

Xu ZH, 1999, APPL ENVIRON MICROB, V65, P5142

[60]

SELECTED LYMPHOCYTE

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