A 1H NMR-based metabonomic study on the SAMP8 and SAMR1 mice and the effect of electro-acupuncture

被引：45

作者：

Wu Qiao-feng ^{[1
]}

Guo Ling-ling ^{[2
]}

Yu Shu-guang ^{[1
]}

Zhang Qi ^{[3
]}

Lu Sheng-feng ^{[1
]}

Zeng Fang ^{[1
]}

Yin Hai-yan ^{[1
]}

Tang Yong ^{[1
]}

Yan Xian-zhong ^{[3
]}

机构：

[1] Chengdu Univ Tradit Chinese Medcine, Acupuncture & Moxibust Coll, Chengdu 610075, Sichuan, Peoples R China

[2] Cent Hosp Zibo, Zibo 255202, Shandong, Peoples R China

[3] Natl Ctr Biomed Anal, Beijing 100850, Peoples R China

来源：

EXPERIMENTAL GERONTOLOGY | 2011年 / 46卷 / 10期

基金：

中国国家自然科学基金;

关键词：

Acupuncture; Electo-acupuncture; Metabonomics; H-1 nuclear magnetic resonances; Senescence; ORTHOGONAL SIGNAL CORRECTION; H-1-NMR SPECTROSCOPY; LACTATE METABOLISM; BLOOD; ACUPUNCTURE; GLUCOSE; SYSTEMS; URINE; RAT;

D O I：

10.1016/j.exger.2011.06.002

中图分类号：

R592 [老年病学]; C [社会科学总论];

学科分类号：

030301 [社会学]; 100201 [内科学];

摘要：

A H-1 NMR-based metabonomic method was used to investigate the metabolic change of plasma in senescence-prone 8 (SAMP8) mice before and after electro-acupuncture (EA). Sixteen SAMP8 male mice (aged 8 months) were randomly divided into model group and acupuncture treatment group while the later group received EA treatment for 21 days. Eight senescence-resistant 1 (SAMR1) mice were used as the control group. Morris water maze was used to evaluate the effects of EA. All mice plasma samples obtained from different groups were analyzed by using 600 MHz H-1 nuclear magnetic resonances (H-1 NMR) spectroscopy. The data sets were analyzed by Principal Components Analysis (PCA) and Partial Least Squares-Discriminant Analysis (PLS-DA) to discriminate the key plasma metabolites among different groups. Results indicated that both the escape and probe tasks of SAMP8 could be improved by EA treatment. Metabonomic study showed that SAMR1 and SAMP8 were separated clearly in both CPMG_OSC_PLS and LED _OSC_PLS score plots. Interestingly, samples obtained from EA group were distributed closely to SAMR1 group in CPMG_OSC_PLS score plot, but away from SAMP8 group in LED_OSC_PLS score plot. Corresponding loading plots showed that much less lactate was seen in SAMP8 mice plasma. Other changes including higher levels of dimethylamine (DMA) Choline and alpha-glucose but lower levels of leucine/isoleucine, HDL, LDL/VLDL, 3-Hydroxybutyrate (3-HB), and Trimethylamine N-oxide (TMAO) were observed in the SAMP8 mice plasma than in the SAMR1. After EA treatment, the levels of lactate, DMA, choline and TMAO were improved. Results of this work can provide valuable clues to the understanding of the metabolic changes in the senile impairment of mice. It is also hoped that the methodology can be used in evaluating the effects of EA and understanding the underlying acupuncture mechanism in treating neurodegenerative diseases. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：787 / 793

页数：7

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