Functional identification of a fatty acid Δ5 desaturase gene from Caenorhabditis elegans

被引：72

作者：

Michaelson, LV

Napier, JA

Lewis, M

Griffiths, G

Lazarus, CM

Stobart, AK

机构：

[1] Univ Bristol, Sch Biol Sci, Bristol BS8 1UG, Avon, England

[2] Univ Bristol, Dept Agr Sci, IACR Long Ashton Res Stn, Bristol BS41 9AF, Avon, England

[3] Hort Res Int, Warwick CV35 9EF, England

来源：

FEBS LETTERS | 1998年 / 439卷 / 03期

基金：

英国生物技术与生命科学研究理事会;

关键词：

fatty acid desaturase; delta five; Caenorhabditis elegans;

D O I：

10.1016/S0014-5793(98)01385-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have identified a cDNA from the nematode worm Caenorhabditis elegans that encodes a fatty acid Delta(5) desaturase, Saccharomyces cerevisiae expressing the full-length cDNA was able to convert di-homo-gamma-linolenic acid to arachidonic acid, thus confirming Delta(5) desaturation. The 1341 hp Delta(5) desaturase sequence contained an N-terminal cytochrome b(5) domain and was located within a kilobase of the C. elegans Delta(6) desaturase on chromosome IV. With an amino acid identity of 45% it is possible that one of these genes arose from the other by gene duplication. This is the first example of a Delta(5) desaturase gene isolated from an animal. (C) 1998 Federation of European Biochemical Societies.

引用

页码：215 / 218

页数：4

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[1] EICOSANOID SYNTHESIS IN 7,12-DIMETHYLBENZ(A)ANTHRACENE-INDUCED MAMMARY CARCINOMAS IN SPRAGUE-DAWLEY RATS FED PRIMROSE OIL, MENHADEN OIL OR CORN-OIL DIET [J].