Mosquito transcriptome changes and filarial worm resistance in Armigeres subalbatus

被引:28
作者
Aliota, Matthew T. [1 ]
Fuchs, Jeremy F. [1 ]
Mayhew, George F. [1 ]
Chen, Cheng-Chen [2 ]
Christensen, Bruce M. [1 ]
机构
[1] Univ Wisconsin, Dept Pathobiol Sci, Madison, WI 53706 USA
[2] Natl Yang Ming Univ, Dept Trop Med, Taipei 112, Taiwan
关键词
D O I
10.1186/1471-2164-8-463
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background: Armigeres subalbatus is a natural vector of the filarial worm Brugia pahangi, but it rapidly and proficiently kills Brugia malayi microfilariae by melanotic encapsulation. Because B. malayi and B. pahangi are morphologically and biologically similar, the Armigeres-Brugia system serves as a valuable model for studying the resistance mechanisms in mosquito vectors. We have initiated transcriptome profiling studies in Ar. subalbatus to identify molecular components involved in B. malayi refractoriness. Results: These initial studies assessed the transcriptional response of Ar. subalbatus to B. malayi at 1, 3, 6, 12, 24, 48, and 72 hrs after an infective blood feed. In this investigation, we initiated the first holistic study conducted on the anti-filarial worm immune response in order to effectively explore the functional roles of immune-response genes following a natural exposure to the parasite. Studies assessing the transcriptional response revealed the involvement of unknown and conserved unknowns, cytoskeletal and structural components, and stress and immune responsive factors. The data show that the anti-filarial worm immune response by Ar. subalbatus to be a highly complex, tissue-specific process involving varied effector responses working in concert with blood cell-mediated melanization. Conclusion: This initial study provides a foundation and direction for future studies, which will more fully dissect the nature of the anti-filarial worm immune response in this mosquito-parasite system. The study also argues for continued studies with RNA generated from both hemocytes and whole bodies to fully expound the nature of the anti-filarial worm immune response.
引用
收藏
页数:15
相关论文
共 71 条
[31]   Role of dopachrome conversion enzyme in the melanization of filarial worms in mosquitoes [J].
Huang, CY ;
Christensen, BM ;
Chen, CC .
INSECT MOLECULAR BIOLOGY, 2005, 14 (06) :675-682
[32]   Molecular cloning of a second prophenoloxidase cDNA from the mosquito Armigeres subalbatus:: prophenoloxidase expression in blood-fed and microfilariae-inoculated mosquitoes [J].
Huang, LH ;
Christensen, BM ;
Chen, CC .
INSECT MOLECULAR BIOLOGY, 2001, 10 (01) :87-95
[33]   Nature or nurture in mosquito resistance to malaria? [J].
Hurd, Hilary .
TRENDS IN PARASITOLOGY, 2007, 23 (04) :135-138
[34]   The role of phenylalanine hydroxylase in melanotic encapsulation of filarial worrris in two species of mosquitoes [J].
Infanger, LC ;
Rocheleau, TA ;
Bartholomay, LC ;
Johnson, JK ;
Fuchs, J ;
Higgs, S ;
Chen, CC ;
Christensen, BM .
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2004, 34 (12) :1329-1338
[35]   Transgenic anopheline mosquitoes impaired in transmission of a malaria parasite [J].
Ito, J ;
Ghosh, A ;
Moreira, LA ;
Wimmer, EA ;
Jacobs-Lorena, M .
NATURE, 2002, 417 (6887) :452-455
[36]   Identification and molecular characterization of a prophenoloxidase involved in Aedes aegypti chorion melanization [J].
Kim, SR ;
Yao, R ;
Han, Q ;
Christensen, BM ;
Li, J .
INSECT MOLECULAR BIOLOGY, 2005, 14 (02) :185-194
[37]   The role of reactive oxygen species on Plasmodium melanotic encapsulation in Anopheles gambiae [J].
Kumar, S ;
Christophides, GK ;
Cantera, R ;
Charles, B ;
Han, YS ;
Meister, S ;
Dimopoulos, G ;
Kafatos, FC ;
Barillas-Mury, C .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (24) :14139-14144
[38]   Importance of replication in microarray gene expression studies: Statistical methods and evidence from repetitive cDNA hybridizations [J].
Lee, MLT ;
Kuo, FC ;
Whitmore, GA ;
Sklar, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (18) :9834-9839
[39]   DOPACHROME CONVERSION ACTIVITY IN AEDES-AEGYPTI - SIGNIFICANCE DURING MELANOTIC ENCAPSULATION OF PARASITES AND CUTICULAR TANNING [J].
LI, JY ;
ZHAO, XL ;
CHRISTENSEN, BM .
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1994, 24 (10) :1043-1049
[40]   Involvement of peroxidase in chorion hardening in Aedes aegypti [J].
Li, JY ;
Hodgeman, BA ;
Christensen, BM .
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1996, 26 (03) :309-317