BMP2 induces chondrogenic differentiation, osteogenic differentiation and endochondral ossification in stem cells

被引：249

作者：

Zhou, Nian ^{[1
]}

Li, Qi ^{[1
]}

Lin, Xin ^{[1
]}

Hu, Ning ^{[1
]}

Liao, Jun-Yi ^{[1
]}

Lin, Liang-Bo ^{[1
]}

Zhao, Chen ^{[1
]}

Hu, Zhen-Ming ^{[1
]}

Liang, Xi ^{[1
]}

Xu, Wei ^{[1
]}

Chen, Hong ^{[1
]}

Huang, Wei ^{[1
]}

机构：

[1] Chongqing Med Univ, Dept Orthoped Surg, Affiliated Hosp 1, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China

来源：

CELL AND TISSUE RESEARCH | 2016年 / 366卷 / 01期

关键词：

Bone morphogenetic protein 2; Chondrogenic differentiation; Osteogenic differentiation; Cartilage tissue engineering; Stem cell; MULTIPOTENTIAL MESENCHYMAL CELLS; BONE MORPHOGENETIC PROTEIN-2; CHONDROCYTE MATURATION; TRANSCRIPTION FACTOR; SKELETAL DEVELOPMENT; CARTILAGE FORMATION; MICROMASS CULTURES; RUNX2; GROWTH; SOX9;

D O I：

10.1007/s00441-016-2403-0

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Bone morphogenetic protein 2 (BMP2), a member of the transforming growth factor-beta (TGF-beta) super-family, is one of the main chondrogenic growth factors involved in cartilage regeneration. BMP2 is known to induce chondrogenic differentiation in various types of stem cells in vitro. However, BMP2 also induces osteogenic differentiation and endochondral ossification in mesenchymal stem cells (MSCs). Although information regarding BMP2-induced chondrogenic and osteogenic differentiation within the same system might be essential for cartilage tissue engineering, few studies concerning these issues have been conducted. In this study, BMP2 was identified as a regulator of chondrogenic differentiation, osteogenic differentiation and endochondral bone formation within the same system. BMP2 was used to regulate chondrogenic and osteogenic differentiation in stem cells within the same culture system in vitro and in vivo. Any changes in the differentiation markers were assessed. BMP2 was found to induce chondrogenesis and osteogenesis in vitro via the expression of Sox9, Runx2 and its downstream markers. According to the results of the subcutaneous stem cell implantation studies, BMP2 not only induced cartilage formation but also promoted endochondral ossification during ectopic bone/cartilage formation. In fetal limb cultures, BMP2 promoted chondrocyte hypertrophy and endochondral ossification. Our data reveal that BMP2 can spontaneously induce chondrogenic differentiation, osteogenic differentiation and endochondral bone formation within the same system. Thus, BMP2 can be used in cartilage tissue engineering to regulate cartilage formation but has to be properly regulated for cartilage tissue engineering in order to retain the cartilage phenotype.

引用

页码：101 / 111

页数：11

共 40 条

[11]

TGF-β3-induced chondrogenesis in co-cultures of chondrocytes and mesenchymal stem cells on biodegradable scaffolds [J].