Silk fibroin microparticles as carriers for delivery of human recombinant BMPs. Physical characterization and drug release

被引：110

作者：

Bessa, P. C. ^{[1
,2
,3
,4
]}

Balmayor, E. R. ^{[2
,3
]}

Azevedo, H. S. ^{[2
,3
]}

Nuernberger, S. ^{[1
]}

Casal, M. ^{[4
]}

van Griensven, M. ^{[1
]}

Reis, R. L. ^{[2
,3
]}

Redl, H. ^{[1
]}

机构：

[1] Ludwig Boltzmann Inst Expt & Clin Traumatol, AUVA Res Ctr, Austrian Cluster Tissue Regenerat, A-1200 Vienna, Austria

[2] Univ Minho, Res Grp Biomat Biodegradables & Biomimet 3Bs, European Inst Excellence Tissue Engn & Regenerat, P-4806909 Taipas, Guimaraes, Portugal

[3] IBB, PT Associated Lab, Guimaraes, Portugal

[4] Univ Minho, CBMA, Dept Biol, P-4710057 Braga, Portugal

来源：

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE | 2010年 / 4卷 / 05期

关键词：

bone morphogenetic protein; silk fibroin; microparticles; drug delivery; BMP-2; BMP-9; BMP-14; BONE MORPHOGENETIC PROTEIN-2; 3-D SCAFFOLDS; MICROSPHERES; DIFFERENTIATION; EXPRESSION; FACTOR-5; GROWTH; REGENERATION; MATRICES; DEFECTS;

D O I：

10.1002/term.245

中图分类号：

Q813 [细胞工程];

学科分类号：

100113 [医学细胞生物学];

摘要：

Bone morphogenetic proteins (BMPs) are cytokines with strong ability to promote new bone formation. Herein, we report the use of silk fibroin microparticles as carriers for the delivery of BMP-2, BMP-9 or BMP-14. BMP-containing fibroin microparticles were prepared by a mild methodology using dropwise addition of ethanol, exhibiting mean diameters of 2.7 +/- 0.3 mu m. Encapsulation efficiencies varied between 67.9 +/- 6.1 % and 97.7 +/- 2.0 % depending on the type and the amount of BMP loaded. Release kinetics showed that BMP-2, BMP-9 and BMP-14 were released in two phases profile, with a burst release in the first two days followed by a slower release, for a period of 14 days. The release data were best explained by Korsmeyer's model and the Fickian model of drug diffusion. Silk fibroin microparticles can offer a promising approach for the sustained delivery of different BMPs in tissue engineering applications. Copyright (C) 2010 John Wiley & Sons, Ltd.

引用

页码：349 / 355

页数：7

共 40 条

[1]

Bone morphogenetic proteins in tissue engineering: the road from the laboratory to the clinic, part I (basic concepts) [J].