Basophil activation test by flow cytometry: Present and future applications in allergology

被引：139

作者：

Ebo, D. G. ^{[1
]}

Bridts, C. H. ^{[1
]}

Hagendorens, M. M. ^{[1
]}

Aerts, N. E. ^{[1
]}

De Clerck, L. S. ^{[1
]}

Stevens, W. J. ^{[1
]}

机构：

[1] Univ Antwerp, Univ Antwerp Hosp, Fac Med Immunol Allergol Rheumatol, Antwerp, Belgium

来源：

CYTOMETRY PART B-CLINICAL CYTOMETRY | 2008年 / 74B卷 / 04期

关键词：

allergy; basophil; diagnosis; flow cytometry;

D O I：

10.1002/cyto.b.20419

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

The diagnosis of allergic reactions in clinical practice rests upon both clinical history and the demonstration of specific immunoglobulin E (slgE), either in the serum or via skin tests. However, for various reasons, identification of the offending allergen(s) is not always possible. Moreover, not all allergies are IgE-mediated. In an attempt to find reliable methods to investigate hypersensitivity reactions, histamine and sulfidoleukotriene release tests have long been introduced. However, relatively few comprehensive quality reports have been published so far. Upon challenge with a specific allergen, basophils not only secrete quantifiable bioactive mediators but also upregulate the expression of different markers which can be detected efficiently by flow cytometry using specific monoclonal antibodies. This review addresses the principals, particular technical aspects and pitfalls as well as the clinical and research applications of flow-assisted analysis of in vitro activated basophils (C) 2008 Clinical Cytometry Society.

引用

页码：201 / 210

页数：10

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