Different populations of melanocytes are present in hair follicles and epidermis

被引:105
作者
Tobin, DJ [1 ]
Bystryn, JC [1 ]
机构
[1] NYU,SCH MED,RONALD O PERELMAN DEPT DERMATOL,NEW YORK,NY
来源
PIGMENT CELL RESEARCH | 1996年 / 9卷 / 06期
关键词
hair follicle; melanocyte; antibody; antigen; melanin; epidermis; vitiligo; alopecia areata;
D O I
10.1111/j.1600-0749.1996.tb00122.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Melanocytes in human skin reside both in the epidermis and in the matrix and outer root sheath of anagen hair follicles. Comparative study of melanocytes in these different locations has been difficult as hair follicle melanocytes could not be cultured. In this study we used a recently described method of growing hair follicle melanocytes to characterize and compare hair follicle and epidermal melanocytes in the scalp of the same individual. Three morphologically and antigenically distinct types of melanocytes were observed in primary culture. These included (1) moderately pigmented and polydendritic melanocytes derived from epidermis; (2) small, bipolar, amelanotic melanocytes; and (3) large, intensely pigmented melanocytes; the latter two were derived from hair follicles. The three sub-populations of cells all reacted with melanocyte-specific monoclonal antibody. Epidermal and amelanotic hair follicle melanocytes proliferated well in culture, whereas the intensely pigmented hair follicle melanocytes did not. Amelanotic hair follicle melanocytes differed from epidermal melanocytes in being less differentiated, and they expressed less mature melanosome antigens. In addition, hair follicle melanocytes expressed some antigens associated with aIopecia areata, but not antigens associated with vitiligo, whereas the reverse was true for epidermal melanocytes. Thus, antigenically different populations of melanocytes are present in epidermis and hair follicle. This could account for the preferential destruction of hair follicle melanocytes in alopecia areata and of epidermal melanocytes in vitiligo.
引用
收藏
页码:304 / 310
页数:7
相关论文
共 14 条
[1]  
AKUTSU Y, 1986, CANCER RES, V46, P2906
[2]   MONOCLONAL-ANTIBODY TO A HIGHLY GLYCOSYLATED PROTEIN REACTS IN FIXED TISSUE WITH MELANOMA AND OTHER TUMORS [J].
ATKINSON, B ;
ERNST, CS ;
GHRIST, BFD ;
ROSS, AH ;
CLARK, WH ;
HERLYN, M ;
HERLYN, D ;
MAUL, G ;
STEPLEWSKI, Z ;
KOPROWSKI, H .
HYBRIDOMA, 1985, 4 (03) :243-255
[3]   ROLE OF HAIR-FOLLICLES IN THE REPIGMENTATION OF VITILIGO [J].
CUI, J ;
SHEN, LY ;
WANG, GC .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1991, 97 (03) :410-416
[4]  
DER J, 1989, P 2 INT C MEL, P214
[5]  
FULLER BB, 1988, J BIOL CHEM, V262, P4024
[6]   DOPA-negative melanocytes in the outer root sheath of human hair follicles express premelanosomal antigens but not a melanosomal antigen or the melanosome-associated glycoproteins tyrosinase, TRP-1, and TRP-2 [J].
Horikawa, T ;
Norris, DA ;
Johnson, TW ;
Zekman, T ;
Dunscomb, N ;
Bennion, SD ;
Jackson, RL ;
Morelli, JG .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1996, 106 (01) :28-35
[7]   12-O-TETRADECANOYLPHORBOL-13-ACETATE NOT ONLY MODULATES PROLIFERATION RATES, BUT ALSO ALTERS ANTIGEN EXPRESSION AND LAK-CELL SUSCEPTIBILITY OF NORMAL HUMAN MELANOCYTES INVITRO [J].
KRASAGAKIS, K ;
GARBE, C ;
KRUGERKRASAGAKES, S ;
ORFANOS, CE .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1993, 100 (05) :653-659
[8]   ANTIGENS OF MELANOCYTES AND MELANOMA [J].
LYNCH, SA ;
BOUCHARD, BN ;
VIJAYASARADHI, S ;
YUASA, H ;
HOUGHTON, AN .
CANCER AND METASTASIS REVIEWS, 1991, 10 (02) :141-150
[9]   SUPPRESSION OF HUMAN-MELANOMA CELL-GROWTH AND METASTASIS BY THE MELANOMA-ASSOCIATED ANTIGEN CD63 (ME491) [J].
RADFORD, KJ ;
MALLESCH, J ;
HERSEY, P .
INTERNATIONAL JOURNAL OF CANCER, 1995, 62 (05) :631-635
[10]  
STARICCO RG, 1962, J INVEST DERMATOL, V36, P163