Characterisation of ISAV proteins from cell culture

被引：18

作者：

Griffiths, S ^{[1
]}

Cook, M ^{[1
]}

Mallory, B ^{[1
]}

Ritchie, R ^{[1
]}

机构：

[1] Mol Grp RPC, Fredericton, NB E3B 6Z9, Canada

来源：

DISEASES OF AQUATIC ORGANISMS | 2001年 / 45卷 / 01期

关键词：

SAV; proteins; electrophoresis; glycoprotein;

D O I：

10.3354/dao045019

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The characterisation of 2 infectious salmon anemia virus (ISAV) proteins is described. Proteins were harvested from ISAV-infected Chinook salmon embryo (CHSE)-214 cell culture by continuous elution denaturing gel electrophoresis, enabling the harvest of specific molecular weight fractions. Through the use of a polyclonal antiserum to ISAV, it was possible to identify a potentially autolytic major antigen of 72 kDa and a glycosylated protein of approximately 38 kDa which Varied in size depending on cell line compatibility. N-terminal amino acid sequencing of the glycosylated proteins suggests that it is encoded by segment 6 of the ISAV genome. Further, sequence analysis of the glycosylated protein account for the variable molecular weight and may explain differences in host cell compatibility.

引用

页码：19 / 24

页数：6