Covalent coupling of immunoglobulin G to a poly(vinyl)alcohol poly(acrylic acid) graft polymer as a method for fabricating the interfacial-recognition layer of a surface plasmon resonance immunosensor

被引：25

作者：

Disley, DM ^{[1
]}

Blyth, J ^{[1
]}

Cullen, DC ^{[1
]}

You, HX ^{[1
]}

Eapen, S ^{[1
]}

Lowe, CR ^{[1
]}

机构：

[1] Univ Cambridge, Inst Biotechnol, Cambridge CB2 1QT, England

来源：

BIOSENSORS & BIOELECTRONICS | 1998年 / 13卷 / 3-4期

关键词：

atomic force microscopy; covalent coupling; detection limit; immunoglobulin; immunosensor; interfacial-recognition layer; poly(acrylic acid); poly(vinyl)alcohol; surface plasmon resonance; sensitivity;

D O I：

10.1016/S0956-5663(97)00114-0

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The synthesis of a terminally thiolated poly(vinyl)alcohol (PVA) grafted with Poly (acrylic acid) (PAA) side chains is described. The PVA-PAA graft polymer (PVA(g)) was end-tethered to silver surfaces via the terminal thiol functionality and the resultant mobile, hydrophilic polymer matrix exploited for the covalent immobilization of large quantities of polyclonal goat (anti-hIgG) antibody (IgG) with low levels of non-specific adsorption. An SPR immunosensor, fabricated with an IgG-PVA-silver interfacial layer proved capable of performing a sensitive label-free assay of human IgG antigen (hIgG) with minimal non-specific binding interference. A detection limit (D-L) for hIgG from serum of 0.8 mu g/ml (5 nM) and an assay sensitivity of 0.66 ng hIgG/mm(2)/nM are reported. (C) 1998 Elsevier Science S.A. All rights reserved.

引用

页码：383 / 396

页数：14

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