Evaluation of a new chromogenic medium for the detection of methicillin-resistant Staphylococcus aureus carriage on nasal and perianal specimens

被引:10
作者
Reyes, Romina C. [1 ,2 ]
Stoakes, Luba [3 ]
Milburn, Sue [3 ]
Lennox, Gwen [3 ]
Daniel, Janis [3 ]
Silver, Shannon N. [3 ]
Wijnker, E. [4 ]
John, Michael A. [2 ,3 ]
Hussain, Zafar [2 ,3 ]
机构
[1] Univ Western Ontario, Schulich Sch Med & Dent, London, ON N6A 5C1, Canada
[2] London Hlth Sci Ctr, London, ON N6A 5W9, Canada
[3] London Hlth Sci Ctr, Dept Microbiol & Infect Control, London, ON N6A 5W9, Canada
[4] Univ Western Ontario, London, ON N6A 5C1, Canada
关键词
MRSA; chromogenic; screening; infection control; nosocomial infections;
D O I
10.1016/j.diagmicrobio.2007.09.011
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
A new chromogenic medium, denim blue (DB), was compared with methicillin-resistant Staphylococcus aureus (MRSA) Select (MRSAS) for detection of MRSA from surveillance specimens. On DB, MRSA colonies are larger (0.57-0.8 versus 0.45-0.6 mm at 18 and 24 h, respectively). Despite this, sensitivities of DB were 77% and 96% at 18 and 24 h, respectively, and those of MRSAS were 63% and 97.5%. Specificities were significantly higher for MRSAS than DB. The study demonstrates that DB and MRSA are equal for the detection of MRSA from surveillance specimens provided the plates are read after 24 h of incubation. Positive predictive values of both media were less than 95% requiring confirmation of MRSA, by another method, from all new patients. (C) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码:225 / 227
页数:3
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