Carboxylation of epoxides to beta-keto acids in cell extracts of Xanthobacter strain Py2

被引:45
作者
Allen, JR [1 ]
Ensign, SA [1 ]
机构
[1] UTAH STATE UNIV,DEPT CHEM & BIOCHEM,LOGAN,UT 84322
关键词
D O I
10.1128/jb.178.5.1469-1472.1996
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A novel enzymatic reaction involved in the metabolism of aliphatic epoxides by Xanthobacter strain Py2 is described, Cell extracts catalyzed the CO2-dependent carboxylation of propylene oxide (epoxypropane) to form acetoacetate and beta-hydroxybutyrate. The time courses of acetoacetate and beta-hydroxybutyrate formation indicate that acetoacetate is the primary product of propylene oxide carboxylation and that beta-hydroxybutyrate is a secondary product formed by the reduction of acetoacetate, Analogous C-5 carboxylation products were identified,vith 1,2-epoxybutane as the substrate. In the absence of CO2, propylene oxide and 1,2-epoxybutane were isomerized to form acetone and methyl ethyl ketone, respectively, as dead-end products, The carboxylation of short-chain epoxides to beta-keto acids is proposed to serve as the physiological reaction for the metabolism of aliphatic epoxides in Xanthobacter strain Py2.
引用
收藏
页码:1469 / 1472
页数:4
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