A CREB site in the BRCA1 proximal promoter acts as a constitutive transcriptional element

被引：41

作者：

Atlas, E ^{[1
]}

Stramwasser, M ^{[1
]}

Mueller, CR ^{[1
]}

机构：

[1] Queens Univ, Dept Biochem, Canc Res Labs, Kingston, ON K7L 3N6, Canada

来源：

ONCOGENE | 2001年 / 20卷 / 48期

关键词：

BRCA1; expression; transcription; CREB; sporadic breast cancer;

D O I：

10.1038/sj.onc.1204890

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transcriptional regulation of the BRCA1 proximal promoter has been suggested to play a role in the decreased expression of BRCA1 observed in sporadic breast cancer. Computer analysis of the sequence of the proximal promoter reveals the presence of a potential CREB site. We have identified CREB/ATF-1 as the factor interacting with this site in nuclear extracts from MCF-7 and T-47D cells. This site is shown to be important for the constitutive expression of the promoter in these cells, as well as in Hep G2 cells. Despite the presence of this site, the BRCA1 promoter is not responsive to cAMP induction. It appears that CREB acts as a constitutive positive element for BRCA1 expression and that any mechanism inactivating CREB function would have a dramatic effect on BRCA1 expression.

引用

页码：7110 / 7114

页数：5

共 38 条

[11] BRCA1 MUTATIONS IN PRIMARY BREAST AND OVARIAN CARCINOMAS [J].

FUTREAL, PA ;

LIU, QY ;

SHATTUCKEIDENS, D ;

COCHRAN, C ;

HARSHMAN, K ;

TAVTIGIAN, S ;

BENNETT, LM ;

HAUGENSTRANO, A ;

SWENSEN, J ;

MIKI, Y ;

EDDINGTON, K ;

MCCLURE, M ;

FRYE, C ;

WEAVERFELDHAUS, J ;

DING, W ;

GHOLAMI, Z ;

SODERKVIST, P ;

TERRY, L ;

JHANWAR, S ;

BERCHUCK, A ;

IGLEHART, JD ;

MARKS, J ;

BALLINGER, DG ;

BARRETT, JC ;

SKOLNICK, MH ;

KAMB, A ;

WISEMAN, R .

SCIENCE, 1994, 266 (5182) :120-122

[12] CROSS-FAMILY DIMERIZATION OF TRANSCRIPTION FACTORS FOS JUN AND ATF CREB ALTERS DNA-BINDING SPECIFICITY [J].

HAI, T ;

CURRAN, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (09) :3720-3724

[13]

Husain A, 1998, CANCER RES, V58, P1120

[14] Synergism of the ATF/CRE site and GC box in the housekeeping Na,K-ATPase α1 subunit gene is essential for constitutive expression [J].

Kobayashi, M ;

Kawakami, K .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 241 (01) :169-174

[15] RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent ubiquitination [J].

Lorick, KL ;

Jensen, JP ;

Fang, SY ;

Ong, AM ;

Hatakeyama, S ;

Weissman, AM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (20) :11364-11369

[16] Down-regulation of BRCA1 in human sporadic breast cancer; analysis of DNA methylation patterns of the putative promoter region [J].

Magdinier, F ;

Ribieras, S ;

Lenoir, GM ;

Frappart, L ;

Dante, R .

ONCOGENE, 1998, 17 (24) :3169-3176

[17] Regional methylation of the 5′ end CpG island of BRCA1 is associated with reduced gene expression in human somatic cells [J].

Magdinier, F ;

Billard, LM ;

Wittmann, G ;

Frappart, L ;

Benchaïb, M ;

Lenoir, GM ;

Guérin, JF ;

Dante, R .

FASEB JOURNAL, 2000, 14 (11) :1585-1594

[18] CPG methylation within the 5′ regulatory region of the BRCA1 gene is tumor specific and includes a putative CREB binding site [J].

Mancini, DN ;

Rodenhiser, DI ;

Ainsworth, PJ ;

O'Malley, FP ;

Singh, SM ;

Xing, WR ;

Archer, TK .

ONCOGENE, 1998, 16 (09) :1161-1169

[19] SOMATIC MUTATIONS IN THE BRCA1 GENE IN SPORADIC OVARIAN-TUMORS [J].

MERAJVER, SD ;

PHAM, TM ;

CADUFF, RF ;

CHEN, M ;

POY, EL ;

COONEY, KA ;

WEBER, BL ;

COLLINS, FS ;

JOHNSTON, C ;

FRANK, TS .

NATURE GENETICS, 1995, 9 (04) :439-443

[20] A STRONG CANDIDATE FOR THE BREAST AND OVARIAN-CANCER SUSCEPTIBILITY GENE BRCA1 [J].

MIKI, Y ;

SWENSEN, J ;

SHATTUCKEIDENS, D ;

FUTREAL, PA ;

HARSHMAN, K ;

TAVTIGIAN, S ;

LIU, QY ;

COCHRAN, C ;

BENNETT, LM ;

DING, W ;

BELL, R ;

ROSENTHAL, J ;

HUSSEY, C ;

TRAN, T ;

MCCLURE, M ;

FRYE, C ;

HATTIER, T ;

PHELPS, R ;

HAUGENSTRANO, A ;

KATCHER, H ;

YAKUMO, K ;

GHOLAMI, Z ;

SHAFFER, D ;

STONE, S ;

BAYER, S ;

WRAY, C ;

BOGDEN, R ;

DAYANANTH, P ;

WARD, J ;

TONIN, P ;

NAROD, S ;

BRISTOW, PK ;

NORRIS, FH ;

HELVERING, L ;

MORRISON, P ;

ROSTECK, P ;

LAI, M ;

BARRETT, JC ;

LEWIS, C ;

NEUHAUSEN, S ;

CANNONALBRIGHT, L ;

GOLDGAR, D ;

WISEMAN, R ;

KAMB, A ;

SKOLNICK, MH .

SCIENCE, 1994, 266 (5182) :66-71

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