Complete inversion of enantioselectivity towards acetylated tertiary alcohols by a double mutant of a Bacillus subtilis esterase

被引：119

作者：

Bartsch, Sebastian ^{[1
]}

Kourist, Robert ^{[1
]}

Bornscheuer, Uwe T. ^{[1
]}

机构：

[1] Ernst Moritz Arndt Univ Greifswald, Inst Biochem, Dept Biotechnol & Enzyme Catalysis, D-17487 Greifswald, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 08期

关键词：

alcohols; directed evolution; enantioselectivity; enzymes; esterases;

D O I：

10.1002/anie.200704606

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Chemical Equation Presented) Simultaneous saturation mutagenesis at three amino acid residues of esterase BS2 followed by high-throughput screening identified a double mutant (E188W/M193C) with inverted enantiopreference, high E values, and broadened substrate range compared to the wild-type (WT) enzyme, while the single mutants lacked this property. The kinetic resolution of ester 1 with the enzymes is shown in the scheme. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：1508 / 1511

页数：4

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