Speciation analysis for organotin compounds in biomaterials after integrated dissolution, extraction, and derivatization in a focused microwave field

Hydrolysis with acetic acid carried out in a low-power focused microwave field in the presence of sodium tetraethylborate and nonane is shown to shorten the sample preparation time for gas chromatographic determination of organotin compounds in biological materials. After a 3-min reaction time, ethyl derivatives of mono-, di-, and tributyltin, and triphenyltin are quantitatively (>95%) found in the supernatant organic phase that is injected onto a capillary GC column, Two rapid one-step analytical procedures, using flame photometric and atomic emission detection, respectively, were developed on this basis and validated by analyzing the NIES 11 certified reference fish tissue.