Unlocking the potential of metagenomics through replicated experimental design

被引：188

作者：

Knight, Rob ^{[1
,2
]}

Jansson, Janet ^{[3
,4
,5
]}

Field, Dawn ^{[6
]}

Fierer, Noah ^{[7
]}

Desai, Narayan ^{[8
]}

Fuhrman, Jed A. ^{[9
]}

Hugenholtz, Phil ^{[10
,11
]}

van der Lelie, Daniel ^{[12
]}

Meyer, Folker ^{[8
,13
]}

Stevens, Rick ^{[8
,13
]}

Bailey, Mark J. ^{[6
]}

Gordon, Jeffrey I. ^{[14
]}

Kowalchuk, George A. ^{[15
,16
]}

Gilbert, Jack A. ^{[8
,17
]}

机构：

[1] Univ Colorado, Howard Hughes Med Inst, Boulder, CO 80309 USA

[2] Univ Colorado, Dept Chem & Biochem, Boulder, CO 80309 USA

[3] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Earth Sci, Berkeley, CA 94720 USA

[4] Lawrence Berkeley Natl Lab, Joint Genome Inst, Walnut Creek, CA USA

[5] Joint Bioenergy Inst, Emeryville, CA USA

[6] Ctr Ecol & Hydrol, Oxford, England

[7] Univ Colorado, Dept Ecol & Evolutionary Biol, Cooperat Inst Res Environm Sci, Boulder, CO 80309 USA

[8] Argonne Natl Lab, Argonne, IL 60439 USA

[9] Univ So Calif, Dept Biol Sci, Los Angeles, CA 90089 USA

[10] Univ Queensland, Australian Ctr Ecogen, Sch Chem & Mol Biosci, St Lucia, Qld, Australia

[11] Univ Queensland, Inst Mol Biosci, St Lucia, Qld, Australia

[12] RTI, Durham, NC USA

[13] Univ Chicago, Computat Inst, Chicago, IL 60637 USA

[14] Washington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO USA

[15] Netherlands Inst Ecol NIOO KNAW, Wageningen, Netherlands

[16] Vrije Univ Amsterdam, Inst Ecol Sci, Amsterdam, Netherlands

[17] Univ Chicago, Dept Ecol & Evolut, Chicago, IL 60637 USA

来源：

NATURE BIOTECHNOLOGY | 2012年 / 30卷 / 06期

基金：

美国国家卫生研究院; 美国国家科学基金会; 英国自然环境研究理事会;

关键词：

COMPARATIVE-ANALYSIS SYSTEM; WESTERN ENGLISH-CHANNEL; GUT MICROBIOME; FUNCTIONAL-ANALYSIS; GLOBAL PATTERNS; BACTERIAL; COMMUNITIES; REVEALS; GENOMES; ENTEROTYPES;

D O I：

10.1038/nbt.2235

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Metagenomics holds enormous promise for discovering novel enzymes and organisms that are biomarkers or drivers of processes relevant to disease, industry and the environment. In the past two years, we have seen a paradigm shift in metagenomics to the application of cross-sectional and longitudinal studies enabled by advances in DNA sequencing and high-performance computing. These technologies now make it possible to broadly assess microbial diversity and function, allowing systematic investigation of the largely unexplored frontier of microbial life. To achieve this aim, the global scientific community must collaborate and agree upon common objectives and data standards to enable comparative research across the Earth's microbiome. Improvements in comparability of data will facilitate the study of biotechnologically relevant processes, such as bioprospecting for new glycoside hydrolases or identifying novel energy sources.

引用

页码：513 / 520

页数：8

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