Identification of substrains of BCG vaccine using multiplex PCR

被引：59

作者：

Bedwell, J ^{[1
]}

Kairo, SK ^{[1
]}

Behr, MA ^{[1
]}

Bygraves, JA ^{[1
]}

机构：

[1] McGill Univ, Ctr Hlth, Dept Med, Div Infect Dis, Montreal, PQ H3G 1A4, Canada

来源：

VACCINE | 2001年 / 19卷 / 15-16期

关键词：

BCG vaccine; identity; PCR;

D O I：

10.1016/S0264-410X(00)00369-8

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Current methods for determining the identity of substrains of Mycobacterium bovis BCG (BCG) vaccine are labour intensive, or provide only limited substrain differentiation. In this paper we describe a multiplex PCR that distinguishes between M. tuberculosis (TB) and M. bovis and the non-pathogenic BCG strain, and also subdivides the BCG vaccine substrains investigated into seven distinct fingerprints based on six target regions in the DNA. This test is specific, rapid, reproducible and portable and is proposed as a novel test for BCG vaccine control. It offers substantial advantages over the methods currently in use. Using this test we have characterised a number of commercial BCG vaccines. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：2146 / 2151

页数：6