Liquid chromatographic determination of myocardial interstitial epinephrine

被引：26

作者：

Kawada, T

Yamazaki, T

Akiyama, T

Sato, T

Shishido, T

Sugimachi, M

Inagaki, M

Alexander , J

Sunagawa, K

机构：

[1] Natl Cardiovasc Ctr, Res Inst, Dept Cardiovasc Dynam, Suita, Osaka 565, Japan

[2] Natl Cardiovasc Ctr, Res Inst, Dept Cardiac Physiol, Suita, Osaka 565, Japan

[3] Vanderbilt Univ, Dept Biomed Engn, Nashville, TN 37235 USA

来源：

JOURNAL OF CHROMATOGRAPHY B | 1998年 / 714卷 / 02期

基金：

日本科学技术振兴机构;

关键词：

epinephrine;

D O I：

10.1016/S0378-4347(98)00221-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This study describes a high-performance liquid chromatographic method with electrochemical detection (HPLC-ED) for monitoring of epinephrine (Epi) in the myocardial interstitial space. The in vitro detection limit for Epi was 200 fg in a 50-mu l injection. Using a cardiac dialysis technique, 60-mu l dialysates were sampled from the myocardial interstitial space (6-min fractions). After an alumina procedure, the dialysate Epi concentration was measured using the HPLC-ED system. Although the basal Epi concentration was undetectable, local administration of desipramine increased Epi concentration of the dialysate to 38.1 +/- 18.5 pg/ml. This system affords a new possibility for estimating myocardial interstitial Epi level. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

收藏

页码：375 / 378

页数：4

相关论文

共 10 条

[1] INVIVO MONITORING OF MYOCARDIAL INTERSTITIAL NOREPINEPHRINE BY DIALYSIS TECHNIQUE [J].

AKIYAMA, T ;

YAMAZAKI, T ;

NINOMIYA, I .

AMERICAN JOURNAL OF PHYSIOLOGY, 1991, 261 (05) :H1643-H1647

[2] DISPOSITION OF ENDOGENOUS ADRENALINE COMPARED TO NORADRENALINE RELEASED BY CARDIAC SYMPATHETIC-NERVES IN THE ANESTHETIZED DOG [J].

EISENHOFER, G ;

SMOLICH, JJ ;

ESLER, MD .

NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 1992, 345 (02) :160-171

[3] EFFECTS OF DESIPRAMINE ON SYMPATHETIC-NERVE FIRING AND NOREPINEPHRINE SPILLOVER TO PLASMA IN HUMANS [J].

ESLER, MD ;

WALLIN, G ;

DORWARD, PK ;

EISENHOFER, G ;

WESTERMAN, R ;

MEREDITH, I ;

LAMBERT, G ;

COX, HS ;

JENNINGS, G .

AMERICAN JOURNAL OF PHYSIOLOGY, 1991, 260 (04) :R817-R823

[4] DESIPRAMINE-INDUCED INCREASE IN EFFLUX OF ENDOGENOUS DOPAMINE FROM RAT HYPOTHALAMUS INVITRO - MECHANISM AND SPECIFICITY [J].

MYERS, SL ;

TESSEL, RE .

NEUROPHARMACOLOGY, 1983, 22 (05) :601-607

[5]

PAPKA RE, 1974, CELL TISSUE RES, V154, P471

[6] EFFECT OF CHANGES IN MYOCARDIAL EPINEPHRINE STORES ON PLASMA NOREPINEPHRINE GRADIENT ACROSS THE DOG HEART [J].

PERONNET, F ;

BOUDREAU, G ;

DECHAMPLAIN, J ;

NADEAU, R .

AMERICAN JOURNAL OF PHYSIOLOGY, 1994, 266 (06) :H2404-H2409

[7] Left ventricular norepinephrine and epinephrine kinetics at birth in lambs [J].

Smolich, JJ ;

Cox, HS ;

Berger, PJ ;

Walker, AM ;

Eisenhofer, G ;

Eisler, MD .

CIRCULATION RESEARCH, 1997, 81 (03) :438-447

[8] High-performance liquid chromatographic determination of myocardial interstitial dihydroxyphenylglycol [J].

Takauchi, Y ;

Kitagawa, H ;

Kawada, T ;

Akiyama, T ;

Yamazaki, T .

JOURNAL OF CHROMATOGRAPHY B, 1997, 693 (01) :218-221

[9] A new, concise dialysis approach to assessment of cardiac sympathetic nerve terminal abnormalities [J].

Yamazaki, T ;

Akiyama, T ;

Kitagawa, H ;

Takauchi, Y ;

Kawada, T ;

Sunagawa, K .

AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 1997, 272 (03) :H1182-H1187

[10] omega-conotoxin GVIA and desipramine insensitive norepinephrine efflux from cardiac sympathetic nerve terminal [J].

Yamazaki, T ;

Kawada, T ;

Akiyama, T ;

Kitagawa, H ;

Takauchi, Y ;

Yahagi, N ;

Sunagawa, K .

BRAIN RESEARCH, 1997, 761 (02) :329-332